desulfuromonas_medium_tce

CultureMech:001868 bacterial COMPLEX LIQUID
Original name: DESULFUROMONAS MEDIUM (TCE)

Overview

External ontology
mediadive.medium:732a (source link unavailable) — DESULFUROMONAS MEDIUM (TCE)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'dehalobacter_restrictus_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:732', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_13726', 'source': 'KOMODO', 'source_id': 'komodo.medium:732.1', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_15941', 'source': 'KOMODO', 'source_id': 'komodo.medium:732.2', 'original_category': 'bacterial'}
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium732a.pdf

Composition

flowchart LR
    CultureMech_001868["desulfuromonas_medium_tce"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001868 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_001868 --> ing1_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing1_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing2_Peptone["Peptone"]
    CultureMech_001868 --> ing2_Peptone
    ing3_Na_acetate["Na-acetate"]
    CultureMech_001868 --> ing3_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Na_acetate -.-> CHEBI_32954
    ing4_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001868 --> ing4_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Sodium_resazurin -.-> CHEBI_8806
    ing5__NH4_2CO3["(NH4)2CO3"]
    CultureMech_001868 --> ing5__NH4_2CO3
    ing6_NaHCO3["NaHCO3"]
    CultureMech_001868 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001868 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001868 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001868 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Hexadecane["Hexadecane"]
    CultureMech_001868 --> ing10_Hexadecane
    CHEBI_45296["CHEBI:45296"]
    ing10_Hexadecane -.-> CHEBI_45296
    ing11_Tetrachloroethene["Tetrachloroethene"]
    CultureMech_001868 --> ing11_Tetrachloroethene
    CHEBI_17300["CHEBI:17300"]
    ing11_Tetrachloroethene -.-> CHEBI_17300
    ing12_NaOH["NaOH"]
    CultureMech_001868 --> ing12_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing12_NaOH -.-> CHEBI_32145
    ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001868 --> ing13_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing14_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001868 --> ing14_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing14_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing15_Na2_EDTA["Na2-EDTA"]
    CultureMech_001868 --> ing15_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing15_Na2_EDTA -.-> CHEBI_64734
    ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001868 --> ing16_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing17_ZnCl2["ZnCl2"]
    CultureMech_001868 --> ing17_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing17_ZnCl2 -.-> CHEBI_49976
    ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001868 --> ing18_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing19_H3BO3["H3BO3"]
    CultureMech_001868 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001868 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001868 --> ing21_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing22_AlCl3["AlCl3"]
    CultureMech_001868 --> ing22_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing22_AlCl3 -.-> CHEBI_30114
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001868 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001868 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_Biotin["Biotin"]
    CultureMech_001868 --> ing25_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing25_Biotin -.-> CHEBI_15956
    ing26_Folic_acid["Folic acid"]
    CultureMech_001868 --> ing26_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing26_Folic_acid -.-> CHEBI_27470
    ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001868 --> ing27_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing28_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001868 --> ing28_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing28_Thiamine_HCl -.-> CHEBI_49105
    ing29_Riboflavin["Riboflavin"]
    CultureMech_001868 --> ing29_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing29_Riboflavin -.-> CHEBI_17015
    CultureMech_001868 --> more["...8 more"]

Ingredients (38)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.747126 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
0.195402 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
3 Peptone 0.114943 G_PER_L
4 Na-acetate
C2H3O2.Na 82.034 g/mol
0.528736 G_PER_L CHEBI:32954
sodium acetate
5 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000574713 G_PER_L CHEBI:8806
Resazurin
6 (NH4)2CO3 2.7 G_PER_L
7 NaHCO3
CHO3.Na 84.006 g/mol
37.3 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
11 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
10 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
30 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Hexadecane
C16H34 226.448 g/mol
13.5 G_PER_L CHEBI:45296
hexadecane
12 Tetrachloroethene
C2Cl4 165.834 g/mol
1.5 G_PER_L CHEBI:17300
tetrachloroethene
13 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
14 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
15 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
16 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
17 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
2 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
18 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
19 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
23 AlCl3
AlCl3 133.341 g/mol
0.01 G_PER_L CHEBI:30114
aluminium trichloride
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
27 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
28 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.4 G_PER_L CHEBI:30961
pyridoxine hydrochloride
 [Merged 2 duplicates: 0.1, 0.3]
29 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
30 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
31 Nicotinic acid
C6H5NO2 123.111 g/mol
0.25 G_PER_L CHEBI:15940
nicotinic acid
 [Merged 2 duplicates: 0.05, 0.2]
32 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
33 Vitamin B12 0.101 G_PER_L CHEBI:176843
vitamin B12
 [Merged 2 duplicates: 0.001, 0.1]
34 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.13 G_PER_L CHEBI:30753
4-aminobenzoic acid
 [Merged 2 duplicates: 0.05, 0.08]
35 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
36 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
37 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
38 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.2 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 9 ml in 50 ml bottles) and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C, D and F are autoclave under 100% N2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Prepare solution G by filling 13.5 ml hexadecane into a 50 ml serum bottle, then sparge with 100% N2 gas to make it anoxic and autoclave. Add 1.50 ml anoxic autoclaved tetrachloroethene to the sterile anoxic hexadecane solution by syringe. To complete the medium add appropriate amounts of solutions B to F to the sterile solution A in the sequence as indicated. The pH of the medium before inoculation should be at 7.2.
  2. Add solution G only after inoculation of the medium!
  3. First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:12.072016Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 732a
2026-02-06T05:10:21.918943Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:53.187324Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:07.951495Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001868
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.332345+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFUROMONAS MEDIUM (TCE)" to "desulfuromonas_medium_tce" for consistent matching
2026-06-05T07:40:58.442663+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.280658+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:29.962324+00:00 recipe-merger Merged 4 duplicate recipes into canonical record Sources: dehalobacter_restrictus_medium, desulfuromonas_medium_tce, for_dsm_13726, for_dsm_15941
2026-08-06T03:05:29.962410+00:00 merge_recipes.py MERGED_RECIPES merged 4 source recipe(s) on fingerprint 127d117c82f6cf23955d92c66357a770ad1c0c6cca1171f3c8c07e004f56bd24