medium_for_treponema_parvum

Overview

External ontology

mediadive.medium:78d (source link unavailable) — MEDIUM FOR TREPONEMA PARVUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78d.pdf

Composition

flowchart LR
    CultureMech_001930["medium_for_treponema_parvum"]
    ing0_Calf_serum["Calf serum"]
    CultureMech_001930 --> ing0_Calf_serum
    ing1_KCl["KCl"]
    CultureMech_001930 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_Vitamin_B12["Vitamin B12"]
    CultureMech_001930 --> ing2_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing2_Vitamin_B12 -.-> CHEBI_176843
    ing3_Pantothenic_acid["Pantothenic acid"]
    CultureMech_001930 --> ing3_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing3_Pantothenic_acid -.-> CHEBI_7916
    ing4_Riboflavin["Riboflavin"]
    CultureMech_001930 --> ing4_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing4_Riboflavin -.-> CHEBI_17015
    ing5_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_001930 --> ing5_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing5_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing6_Biotin["Biotin"]
    CultureMech_001930 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_Folic_acid["Folic acid"]
    CultureMech_001930 --> ing7_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing7_Folic_acid -.-> CHEBI_27470
    ing8_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001930 --> ing8_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing8_Nicotinic_acid -.-> CHEBI_15940
    ing9_Nicotine_amide["Nicotine amide"]
    CultureMech_001930 --> ing9_Nicotine_amide
    CHEBI_17154["CHEBI:17154"]
    ing9_Nicotine_amide -.-> CHEBI_17154
    ing10_alpha_lipoic_acid["alpha-lipoic acid"]
    CultureMech_001930 --> ing10_alpha_lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing10_alpha_lipoic_acid -.-> CHEBI_16494
    ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001930 --> ing11_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing12_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001930 --> ing12_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing12_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing13_L_Histidin["L-Histidin"]
    CultureMech_001930 --> ing13_L_Histidin
    CHEBI_15971["CHEBI:15971"]
    ing13_L_Histidin -.-> CHEBI_15971
    ing14_L_Serin["L-Serin"]
    CultureMech_001930 --> ing14_L_Serin
    CHEBI_17115["CHEBI:17115"]
    ing14_L_Serin -.-> CHEBI_17115
    ing15_L_Glutamine["L-Glutamine"]
    CultureMech_001930 --> ing15_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing15_L_Glutamine -.-> CHEBI_18050
    ing16_Hemin["Hemin"]
    CultureMech_001930 --> ing16_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing16_Hemin -.-> CHEBI_50385
    ing17_NaOH["NaOH"]
    CultureMech_001930 --> ing17_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing17_NaOH -.-> CHEBI_32145
    ing18_Tryptone_peptone["Tryptone peptone"]
    CultureMech_001930 --> ing18_Tryptone_peptone
    ing19_Casamino_acids["Casamino acids"]
    CultureMech_001930 --> ing19_Casamino_acids
    ing20_Yeast_extract["Yeast extract"]
    CultureMech_001930 --> ing20_Yeast_extract
    ing21_Ascorbic_acid["Ascorbic acid"]
    CultureMech_001930 --> ing21_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing21_Ascorbic_acid -.-> CHEBI_22652
    ing22_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_001930 --> ing22_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing22_N_Acetylglucosamine -.-> CHEBI_59640
    ing23_Na_pyruvate["Na-pyruvate"]
    CultureMech_001930 --> ing23_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing23_Na_pyruvate -.-> CHEBI_50144
    ing24_Glutathione["Glutathione"]
    CultureMech_001930 --> ing24_Glutathione
    CHEBI_16856["CHEBI:16856"]
    ing24_Glutathione -.-> CHEBI_16856
    ing25_Ground_beef["Ground beef"]
    CultureMech_001930 --> ing25_Ground_beef
    ing26_Casitone["Casitone"]
    CultureMech_001930 --> ing26_Casitone
    ing27_K2HPO4["K2HPO4"]
    CultureMech_001930 --> ing27_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing27_K2HPO4 -.-> CHEBI_131527
    ing28_Resazurin["Resazurin"]
    CultureMech_001930 --> ing28_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing28_Resazurin -.-> CHEBI_8806
    ing29_L_Cysteine_HCl["L-Cysteine HCl"]
    CultureMech_001930 --> ing29_L_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing29_L_Cysteine_HCl -.-> CHEBI_91247
    CultureMech_001930 --> more["...5 more"]

Ingredients (35)

Preparation

1. To medium 78 add per liter:
2. Prepare medium anaerobically under N2:CO2 (80:20). After inoculation of the tubes, pressurize them with hydrogen gas to 150 kP (1.5 bar).
3. All following solutions were filter sterilized
4. Stir for some hours, filter sterilize the solution.
5. Amino acid solution per 50 ml:
6. Dissolve 5 mg hemin in 1 ml 1 M NaOH, fill up to 50 ml with dist. water.
7. Peptone mixture per 50 ml:
8. Dissolve 1 g ascorbic acid in 40 ml dist. water, adjust pH to 7.0. fill up to 50 ml.
9. N-Acetylglucosamin and pyruvate solution per 50 ml :
10. Dissolve 7 g glutathione in 40 ml dist. Water, adjust pH to 7.0 and fill up to 50 ml.
11. Depending on organism use different modification .
12. To make medium anoxic bring it to a boil, cool under 100% N2 gas atmosphere, add 0.5 g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under 100% N2 gas atmosphere by filling 7 ml medium into anoxic Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121°C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium.
13. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving:
14. Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add:
15. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.
16. Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.
17. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• medium_for_treponema_parvum (CultureMech:001930)