Overview
- External ontology
- mediadive.medium:796 (source link unavailable) — ARCHAEOGLOBUS VENEFICUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium796.pdf
Composition
flowchart LR
CultureMech_001940["archaeoglobus_veneficus_medium"]
ing0_NaCl["NaCl"]
CultureMech_001940 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_001940 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001940 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_NH4Cl["NH4Cl"]
CultureMech_001940 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001940 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001940 --> ing5_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing6_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_001940 --> ing6_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing6_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001940 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_NaHCO3["NaHCO3"]
CultureMech_001940 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Na2SO3["Na2SO3"]
CultureMech_001940 --> ing9_Na2SO3
CHEBI_86477["CHEBI:86477"]
ing9_Na2SO3 -.-> CHEBI_86477
ing10_Na_acetate["Na-acetate"]
CultureMech_001940 --> ing10_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing10_Na_acetate -.-> CHEBI_32954
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001940 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001940 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13__NH4_2Ni_SO4_2["(NH4)2Ni(SO4)2"]
CultureMech_001940 --> ing13__NH4_2Ni_SO4_2
CHEBI_86149["CHEBI:86149"]
ing13__NH4_2Ni_SO4_2 -.-> CHEBI_86149
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001940 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001940 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001940 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001940 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_H3BO3["H3BO3"]
CultureMech_001940 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001940 --> ing19_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001940 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001940 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001940 --> ing22_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
27.9462 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 17.9462, 10.0] |
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.329013 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
7.12861 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.249252 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.139581 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.139581, 1.0] |
| 6 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.179462 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00199402 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000498504 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.98804 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
Na2SO3
2Na.O3S 126.044 g/mol
|
0.997009 G_PER_L |
CHEBI:86477
sodium sulfite
|
|
| 11 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.997009 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.498504 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
5.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
(NH4)2Ni(SO4)2
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.1 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.1 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Dissolve ingredients (except bicarbonate, sulfite, acetate and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Thereafter, add and dissolve bicarbonate and equilibrate the medium with the 80% N2 and 20% CO2 gas mixture to achieve a medium pH of 7.0. Dispense medium under same gas atmosphere into serum vials (e.g., 25 ml per 100 ml bottle) and autoclave. After sterilization add sulfite, acetate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The stock solution of sulfite should be freshly prepared and sterilized by filtration. Prior to inoculation check pH of complete medium and adjust to 7.0, if necessary.
- After inoculation, pressurize bottles to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- Adjust pH to 1.0 with HCl.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- archaeoglobus_veneficus_medium (CultureMech:001940)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.290898Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 796 |
| 2026-01-28T19:48:39.748058Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:38.570768Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:08.669138Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001940 |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.136683+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ARCHAEOGLOBUS VENEFICUS MEDIUM" to "archaeoglobus_veneficus_medium" for consistent matching |
| 2026-06-05T07:40:49.586514+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.486000+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.372935+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 884ff4f1466b6345a733b3760139ea9c5f46c6285b3e4a208348ee695e5ebd0e |