Overview
- External ontology
- mediadive.medium:800 (source link unavailable) — ANAEROBIOSPIRILLUM THOMASII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.9
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium800.pdf
Composition
flowchart LR
CultureMech_001945["anaerobiospirillum_thomasii_medium"]
ing0_Wilkins_Chalgrene_Anaerobe_Broth["Wilkins Chalgrene Anaerobe Broth"]
CultureMech_001945 --> ing0_Wilkins_Chalgrene_Anaerobe_Broth
ing1_5_Aminovaleric_acid["5-Aminovaleric acid"]
CultureMech_001945 --> ing1_5_Aminovaleric_acid
CHEBI_15887["CHEBI:15887"]
ing1_5_Aminovaleric_acid -.-> CHEBI_15887
ing2_Fildes_Enrichment["Fildes Enrichment"]
CultureMech_001945 --> ing2_Fildes_Enrichment
ing3_3_Hydroxybenzoic_acid["3-Hydroxybenzoic acid"]
CultureMech_001945 --> ing3_3_Hydroxybenzoic_acid
CHEBI_30764["CHEBI:30764"]
ing3_3_Hydroxybenzoic_acid -.-> CHEBI_30764
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Wilkins Chalgrene Anaerobe Broth | 10 G_PER_L | ||
| 2 |
5-Aminovaleric acid
C5H11NO2 117.148 g/mol
|
0.5 G_PER_L |
CHEBI:15887
5-aminopentanoic acid
|
|
| 3 | Fildes Enrichment | 1 G_PER_L | ||
| 4 |
3-Hydroxybenzoic acid
C7H6O3 138.122 g/mol
|
0.2 G_PER_L |
CHEBI:30764
3-hydroxybenzoic acid
|
[Merged 2 duplicates: 0.1, 0.1] |
Preparation
- Use Wilkins Chalgrene Anaerobe Broth (Oxoid CM 643) and prepare it anaerobically under 80% N2 + 20% CO2 gas. After autoclaving and cooling to room temperature add per 10 ml the following sterile, anaerobic solutions:
- The pH of the completed medium should be 6.8 - 7.0. For growth an agar plates, Columbia sheep blood agar plates (BBL) may be used and incubated under an anaerobic atmosphere containig 5 - 10% CO2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- anaerobiospirillum_thomasii_medium (CultureMech:001945)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.300974Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 800 |
| 2026-03-13T03:08:43.976609Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:08.704903Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001945 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.175224+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROBIOSPIRILLUM THOMASII MEDIUM" to "anaerobiospirillum_thomasii_medium" for consistent matching |
| 2026-06-05T07:40:48.976609+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:23.505300+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8ca22612bc50f2f0ef4269b5db05a5cb8544e45db28b35973f8773d27b6e1516 |