gracilibacillus_medium

CultureMech:001946 bacterial COMPLEX LIQUID
Original name: GRACILIBACILLUS MEDIUM

Overview

External ontology
mediadive.medium:802 (source link unavailable) — GRACILIBACILLUS MEDIUM
Applications
  • Microbial cultivation
pH
7.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium802.pdf

Composition

flowchart LR
    CultureMech_001946["gracilibacillus_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001946 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001946 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_KCl["KCl"]
    CultureMech_001946 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_NaBr["NaBr"]
    CultureMech_001946 --> ing3_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing3_NaBr -.-> CHEBI_63004
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001946 --> ing4_Yeast_extract
    ing5_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001946 --> ing5_Trypticase_peptone
    ing6_Tris_HCl["Tris-HCl"]
    CultureMech_001946 --> ing6_Tris_HCl
    ing7_KH2PO4["KH2PO4"]
    CultureMech_001946 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_CaCl2["CaCl2"]
    CultureMech_001946 --> ing8_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing8_CaCl2 -.-> CHEBI_3312
    ing9_Glucose["Glucose"]
    CultureMech_001946 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_HCl["HCl"]
    CultureMech_001946 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001946 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001946 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001946 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_ZnCl2["ZnCl2"]
    CultureMech_001946 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_H3BO3["H3BO3"]
    CultureMech_001946 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001946 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001946 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001946 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
    CultureMech_001946 --> ing19_AlCl3_x_6_H2O
    CHEBI_30115["CHEBI:30115"]
    ing19_AlCl3_x_6_H2O -.-> CHEBI_30115
    ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001946 --> ing20_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
4 NaBr
Br.Na 102.894 g/mol
0.1 G_PER_L CHEBI:63004
sodium bromide
5 Yeast extract 2 G_PER_L
6 Trypticase peptone 2 G_PER_L
7 Tris-HCl 12 G_PER_L
8 KH2PO4
H2O4P.K 136.084 g/mol
23.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 CaCl2
Ca.2Cl 110.984 g/mol
5 G_PER_L CHEBI:3312
calcium dichloride
10 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
11 HCl
HCl 36.461 g/mol
3.2 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
21.9802 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 [Merged 2 duplicates: 1.9802, 20.0]
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.247525 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
20.0990099 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Merged 2 duplicates: 0.0990099, 20.0]
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.0693069 G_PER_L CHEBI:49976
zinc dichloride
16 H3BO3
H3BO3 61.833 g/mol
0.00594059 G_PER_L CHEBI:33118
boric acid
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.039604 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0693069 G_PER_L CHEBI:34887
nickel dichloride
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0019802 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
0.0594059 G_PER_L CHEBI:30115
aluminium trichloride hexahydrate
21 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.00594059 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Adjust pH to 7.8 (with NaOH) before autoclaving. To the sterile medium, from sterile stock solutions, add 10 ml KH2PO4 (50 g/l), 5 ml CaCl2 (100 g/l), 2 ml FeCl2/MnCl2 (FeCl2 x 4 H2O, 20 g/l and MnCl2 x 4 H2O, 20 g/l), and glucose to a final concentration of 2 g/l.
  2. Dissolve salts in the HCl in the oder given above, then make up the solution to 1 litre with distilled water.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:12.303512Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 802
2026-02-06T05:10:26.160652Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:59.529875Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:08.713201Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001946
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.183688+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GRACILIBACILLUS MEDIUM" to "gracilibacillus_medium" for consistent matching
2026-06-05T07:41:06.660677+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.162650+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:36.443655+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 42ee192f06a7db13b333b4396396d05755239ba985c55270a911cf47c6fb060e