ss1_medium

CultureMech:001952 bacterial DEFINED SOLID_AGAR
Original name: SS1-MEDIUM

Overview

External ontology
mediadive.medium:808 (source link unavailable) — SS1-MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium808.pdf

Composition

flowchart LR
    CultureMech_001952["ss1_medium"]
    ing0_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_001952 --> ing0_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing0_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001952 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001952 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaNO3["NaNO3"]
    CultureMech_001952 --> ing3_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing3_NaNO3 -.-> CHEBI_63005
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001952 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2["CaCl2"]
    CultureMech_001952 --> ing5_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing5_CaCl2 -.-> CHEBI_3312
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001952 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001952 --> ing7_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing7_MnSO4_x_H2O -.-> CHEBI_86364
    ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001952 --> ing8_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing9_Co_NO3_2["Co(NO3)2"]
    CultureMech_001952 --> ing9_Co_NO3_2
    CHEBI_86209["CHEBI:86209"]
    ing9_Co_NO3_2 -.-> CHEBI_86209
    ing10_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001952 --> ing10_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing10_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001952 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_Methanol["Methanol"]
    CultureMech_001952 --> ing12_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing12_Methanol -.-> CHEBI_17790
    ing13_Agar["Agar"]
    CultureMech_001952 --> ing13_Agar
    CHEBI_2509["CHEBI:2509"]
    ing13_Agar -.-> CHEBI_2509
    ing14_Na_EDTA["Na-EDTA"]
    CultureMech_001952 --> ing14_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing14_Na_EDTA -.-> CHEBI_64734
    ing15_NaOH["NaOH"]
    CultureMech_001952 --> ing15_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing15_NaOH -.-> CHEBI_32145
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001952 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
3.93 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
1.8 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2
Ca.2Cl 110.984 g/mol
0.01 G_PER_L CHEBI:3312
calcium dichloride
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.5 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
8 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.0001 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
9 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0001 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
10 Co(NO3)2
Co.2NO3 182.941 g/mol
0.0001 G_PER_L CHEBI:86209
cobalt dinitrate
11 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0001 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.002 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
14 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
15 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
17.9 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
16 NaOH
HO.Na 39.997 g/mol
3.28 G_PER_L CHEBI:32145
sodium hydroxide
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
13.7 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate

Preparation

  1. pH 6.8
  2. Autoclave the mineral salts solution and add 10 ml/l filter sterilised methanol to the cooled medium. Add 15 g/l agar for solid media.
  3. 1) Dissolve 17.9 g sodium EDTA and 3.28 g NaOH in 186 ml of distilled water.
  4. 2) Dissolve 13.7 g FeSO4 x 7 H2O in 364 ml of distilled ater, bubbling with air overnight, and store in a brown glass bottle.
  5. 3) Combine the two solutions and make up to 1 litre with distilled water.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:12.319774Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 808
2026-03-13T06:19:08.767861Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001952
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.238414+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SS1-MEDIUM" to "ss1_medium" for consistent matching
2026-06-05T07:41:46.844695+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.778528+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:56.515476+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 7520aa2a5b76423552c4b15e95ebe1406b4e11f3ffbf31cb91d04e9f97489de3