Overview
- External ontology
- mediadive.medium:815 (source link unavailable) — DESULFOHALOTOMACULUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium815.pdf
Composition
flowchart LR
CultureMech_001962["desulfohalotomaculum_medium"]
ing0_Na2SO4["Na2SO4"]
CultureMech_001962 --> ing0_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing0_Na2SO4 -.-> CHEBI_32149
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001962 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_NaCl["NaCl"]
CultureMech_001962 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_001962 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NH4Cl["NH4Cl"]
CultureMech_001962 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KH2PO4["KH2PO4"]
CultureMech_001962 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001962 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001962 --> ing7_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing7_SrCl2_x_6_H2O -.-> CHEBI_36385
ing8_MOPS["MOPS"]
CultureMech_001962 --> ing8_MOPS
CHEBI_39074["CHEBI:39074"]
ing8_MOPS -.-> CHEBI_39074
ing9_Yeast_extract["Yeast extract"]
CultureMech_001962 --> ing9_Yeast_extract
ing10_Sodium_resazurin["Sodium resazurin"]
CultureMech_001962 --> ing10_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Sodium_resazurin -.-> CHEBI_8806
ing11_Iron_powder["Iron powder"]
CultureMech_001962 --> ing11_Iron_powder
CHEBI_82664["CHEBI:82664"]
ing11_Iron_powder -.-> CHEBI_82664
ing12_Na_L_lactate["Na-L-lactate"]
CultureMech_001962 --> ing12_Na_L_lactate
CHEBI_232798["CHEBI:232798"]
ing12_Na_L_lactate -.-> CHEBI_232798
ing13_Na2CO3["Na2CO3"]
CultureMech_001962 --> ing13_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing13_Na2CO3 -.-> CHEBI_29377
ing14_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_001962 --> ing14_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing14_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001962 --> ing15_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
ing16_ZnCl2["ZnCl2"]
CultureMech_001962 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001962 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_001962 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001962 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001962 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001962 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001962 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
40 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
8 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.1 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 9 | MOPS | 3 G_PER_L |
CHEBI:39074
MOPS
|
|
| 10 | Yeast extract | 1 G_PER_L | ||
| 11 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Iron powder
Fe 55.845 g/mol
|
100 G_PER_L |
CHEBI:82664
iron(0)
|
|
| 13 |
Na-L-lactate
C3H5O3.Na 112.06 g/mol
|
3.6 G_PER_L |
CHEBI:232798
sodium L-lactate
|
|
| 14 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 15 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 16 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Dissolve ingredients except iron, lactate and carbonate, adjust pH to 6.0 with NaOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing 1 - 2 g iron powder or an iron nail. Autoclave for 30 min at 105°C. Before use add lactate from a sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final medium pH should be 7.2.
- Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfohalotomaculum_medium (CultureMech:001962)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.346368Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 815 |
| 2026-01-28T19:48:35.062214Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:08.855989Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001962 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.341319+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOHALOTOMACULUM MEDIUM" to "desulfohalotomaculum_medium" for consistent matching |
| 2026-06-05T07:40:56.731378+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.625288+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:31.414787+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint be0e757de84c2d6003a94235f6fd8c91ca058aa276facb3cc00e8f85e83690f8 |