Overview
- External ontology
- mediadive.medium:830a (source link unavailable) — MEDIUM R2A MODIFIED
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830a.pdf
Composition
flowchart LR
CultureMech_001985["medium_r2a_modified"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001985 --> ing0_Yeast_extract
ing1_Proteose_peptone["Proteose peptone"]
CultureMech_001985 --> ing1_Proteose_peptone
ing2_Casamino_acids["Casamino acids"]
CultureMech_001985 --> ing2_Casamino_acids
ing3_Glucose["Glucose"]
CultureMech_001985 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Na_pyruvate["Na-pyruvate"]
CultureMech_001985 --> ing4_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Na_pyruvate -.-> CHEBI_50144
ing5_K2HPO4["K2HPO4"]
CultureMech_001985 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001985 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_Agar["Agar"]
CultureMech_001985 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Tween_80["Tween 80"]
CultureMech_001985 --> ing8_Tween_80
CHEBI_53426["CHEBI:53426"]
ing8_Tween_80 -.-> CHEBI_53426
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.5 G_PER_L | ||
| 2 | Proteose peptone | 0.5 G_PER_L | ||
| 3 | Casamino acids | 0.5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 9 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Preparation
- Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_r2a_modified (CultureMech:001985)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.424599Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 830a |
| 2026-01-28T19:48:08.820217Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:09.115613Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001985 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.633791+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM R2A MODIFIED" to "medium_r2a_modified" for consistent matching |
| 2026-06-05T07:41:30.241327+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:47.581139+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:06.494322+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:44.278088+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint eb5faffa7369037d7b2e14f1b702812c6b6ea50b5c4dbd97e15355c6a2c3bd75 |