desulfitobacterium_pcp_1_medium

CultureMech:001993 bacterial COMPLEX LIQUID
Original name: DESULFITOBACTERIUM PCP-1 MEDIUM

Overview

External ontology
mediadive.medium:836 (source link unavailable) — DESULFITOBACTERIUM PCP-1 MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'medium_836_modified_for_dsm_13498', 'source': 'KOMODO', 'source_id': 'komodo.medium:836_13498', 'original_category': 'bacterial'}
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium836.pdf

Composition

flowchart LR
    CultureMech_001993["desulfitobacterium_pcp_1_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001993 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001993 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001993 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001993 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001993 --> ing4_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing4_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001993 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001993 --> ing6_Yeast_extract
    ing7_Na_pyruvate["Na-pyruvate"]
    CultureMech_001993 --> ing7_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing7_Na_pyruvate -.-> CHEBI_50144
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001993 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Na2CO3["Na2CO3"]
    CultureMech_001993 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_Na2SO3["Na2SO3"]
    CultureMech_001993 --> ing10_Na2SO3
    CHEBI_86477["CHEBI:86477"]
    ing10_Na2SO3 -.-> CHEBI_86477
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001993 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_HCl["HCl"]
    CultureMech_001993 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_ZnCl2["ZnCl2"]
    CultureMech_001993 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001993 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_001993 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001993 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001993 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001993 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001993 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.27 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.35 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.53 G_PER_L CHEBI:31206
ammonium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.502 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 [Merged 2 duplicates: 0.002, 1.5]
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Yeast extract 1 G_PER_L
8 Na-pyruvate
C3H3O3.Na 110.044 g/mol
6.05 G_PER_L CHEBI:50144
sodium pyruvate
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
10 Na2CO3
CO3.2Na 105.988 g/mol
0.5 G_PER_L CHEBI:29377
sodium carbonate
11 Na2SO3
2Na.O3S 126.044 g/mol
0.63 G_PER_L CHEBI:86477
sodium sulfite
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.05 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve ingredients (except carbonate, sulfite and sulfide), adjust pH of solution to 7.0 and sparge with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add sulfite and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The stock solution of sulfite should by freshly prepared and sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.5, if necessary.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:12.448451Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 836
2026-03-13T03:09:09.693164Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:09.192222Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001993
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.720126+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFITOBACTERIUM PCP-1 MEDIUM" to "desulfitobacterium_pcp_1_medium" for consistent matching
2026-06-05T07:40:56.050587+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.359299+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:30.301620+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: desulfitobacterium_pcp_1_medium, medium_836_modified_for_dsm_13498
2026-08-06T03:05:30.301671+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 969227ab69e5bd61de6982a68286d98466001456a3075707fee438d282a62208