geoanaerobacter_medium

Overview

External ontology

mediadive.medium:838 (source link unavailable) — GEOANAEROBACTER MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'for_dsm_15288_and_dsm_16228', 'source': 'KOMODO', 'source_id': 'komodo.medium:838.2', 'original_category': 'bacterial'}
• {'name': 'for_dsm_16401', 'source': 'KOMODO', 'source_id': 'komodo.medium:838.3', 'original_category': 'bacterial'}
• {'name': 'for_dsm_19350_and_dsm_22248', 'source': 'KOMODO', 'source_id': 'komodo.medium:838.4', 'original_category': 'bacterial'}
• {'name': 'for_dsm_9736', 'source': 'KOMODO', 'source_id': 'komodo.medium:838.1', 'original_category': 'bacterial'}
• {'name': 'geobacter_dfr2_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:838', 'original_category': 'bacterial'}

pH

6.8

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium838.pdf

Composition

flowchart LR
    CultureMech_001995["geoanaerobacter_medium"]
    ing0_Ferric_citrate["Ferric citrate"]
    CultureMech_001995 --> ing0_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing0_Ferric_citrate -.-> CHEBI_144421
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001995 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001995 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001995 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001995 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Na2CO3["Na2CO3"]
    CultureMech_001995 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Na_acetate["Na-acetate"]
    CultureMech_001995 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na_ascorbate["Na-ascorbate"]
    CultureMech_001995 --> ing7_Na_ascorbate
    CHEBI_113451["CHEBI:113451"]
    ing7_Na_ascorbate -.-> CHEBI_113451
    ing8_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_001995 --> ing8_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing8_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001995 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_ZnCl2["ZnCl2"]
    CultureMech_001995 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001995 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_001995 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001995 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001995 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001995 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001995 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NaOH["NaOH"]
    CultureMech_001995 --> ing17_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing17_NaOH -.-> CHEBI_32145
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001995 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001995 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (20)

Preparation

1. First dissolve ferric citrate by heating the water under continuous stirring. After cooling to room temperature adjust the pH to 6.0, then add and dissolve the remaining ingredients, except carbonate, acetate and ascorbate. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add acetate and ascorbate from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to inoculation check the medium pH and adjust to 6.8, if necessary.
2. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 6 source records.

• for_dsm_15288_and_dsm_16228 (CultureMech:006632)
• for_dsm_16401 (CultureMech:006633)
• for_dsm_19350_and_dsm_22248 (CultureMech:006634)
• for_dsm_9736 (CultureMech:006631)
• geoanaerobacter_medium (CultureMech:001995)
• geobacter_dfr2_medium (CultureMech:006635)