fusibacter_bizertensis_medium

CultureMech:002012 bacterial COMPLEX LIQUID
Original name: FUSIBACTER BIZERTENSIS MEDIUM

Overview

External ontology
mediadive.medium:853b (source link unavailable) — FUSIBACTER BIZERTENSIS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium853b.pdf

Composition

flowchart LR
    CultureMech_002012["fusibacter_bizertensis_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002012 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002012 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002012 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002012 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002012 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_KCl["KCl"]
    CultureMech_002012 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaCl["NaCl"]
    CultureMech_002012 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002012 --> ing7_Yeast_extract
    ing8_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002012 --> ing8_Trypticase_peptone
    ing9_Sodium_resazurin["Sodium resazurin"]
    CultureMech_002012 --> ing9_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Sodium_resazurin -.-> CHEBI_8806
    ing10_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002012 --> ing10_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing10_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002012 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_Na2CO3["Na2CO3"]
    CultureMech_002012 --> ing12_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing12_Na2CO3 -.-> CHEBI_29377
    ing13_D_Glucose["D-Glucose"]
    CultureMech_002012 --> ing13_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing13_D_Glucose -.-> CHEBI_17634
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002012 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002012 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002012 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_002012 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002012 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002012 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002012 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002012 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002012 --> ing22_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing23_H3BO3["H3BO3"]
    CultureMech_002012 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002012 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002012 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002012 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002012 --> ing27_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.990099 G_PER_L CHEBI:31206
ammonium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.29703 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.29703 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.49505 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.19900990000000002 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0990099, 0.1]
6 KCl
Cl.K 74.551 g/mol
0.0990099 G_PER_L CHEBI:32588
potassium chloride
7 NaCl
Cl.Na 58.443 g/mol
3.9703 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 2.9703, 1.0]
8 Yeast extract 0.990099 G_PER_L
9 Trypticase peptone 0.990099 G_PER_L
10 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00049505 G_PER_L CHEBI:8806
Resazurin
11 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
3.12871 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
12 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.49505 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
13 Na2CO3
CO3.2Na 105.988 g/mol
1.48515 G_PER_L CHEBI:29377
sodium carbonate
14 D-Glucose
C6H12O6 180.156 g/mol
3.56436 G_PER_L CHEBI:17634
D-glucose
15 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.29703 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
16 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
17 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
18 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
23 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
24 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
27 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
28 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients, except thiosulfate, cysteine, carbonate, glucose, and sulfide, then sparge medium for 30 - 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Add thiosulfate and cysteine, then adjust pH to 7.0 and dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After autoclaving, add glucose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust the pH of the complete medium to 7.2 - 7.4, if necessary.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:12.512634Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 853b
2026-03-13T03:08:51.700454Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:09.388060Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002012
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.942586+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "FUSIBACTER BIZERTENSIS MEDIUM" to "fusibacter_bizertensis_medium" for consistent matching
2026-05-20T06:01:20.614495+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4}
2026-06-05T07:41:05.683934+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:58.866729+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:35.796712+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint c60d6be69e05430d0b053c0bcaf0a0943bf7d6bc1a83e16bd1051a32cb94527f