desulfotalea_psychrophila_medium

CultureMech:002021 bacterial DEFINED LIQUID
Original name: DESULFOTALEA PSYCHROPHILA MEDIUM

Overview

External ontology
mediadive.medium:861a (source link unavailable) — DESULFOTALEA PSYCHROPHILA MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'desulfofrigus_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:861', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_12341', 'source': 'KOMODO', 'source_id': 'komodo.medium:861.2', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_12342_dsm_12343_and_dsm_12345', 'source': 'KOMODO', 'source_id': 'komodo.medium:861.3', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_12343', 'source': 'KOMODO', 'source_id': 'komodo.medium:861.1', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_12344', 'source': 'KOMODO', 'source_id': 'komodo.medium:861.4', 'original_category': 'bacterial'}
  • {'name': 'srb_psychrophile_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:861', 'original_category': 'bacterial'}
pH range
{'min': 7.0, 'max': 7.2}
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium861a.pdf

Composition

flowchart LR
    CultureMech_002021["desulfotalea_psychrophila_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002021 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2SO4["Na2SO4"]
    CultureMech_002021 --> ing1_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing1_Na2SO4 -.-> CHEBI_32149
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002021 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002021 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002021 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002021 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KBr["KBr"]
    CultureMech_002021 --> ing6_KBr
    CHEBI_32030["CHEBI:32030"]
    ing6_KBr -.-> CHEBI_32030
    ing7_KCl["KCl"]
    CultureMech_002021 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_002021 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Na2CO3["Na2CO3"]
    CultureMech_002021 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_Na_DL_lactate["Na-DL-lactate"]
    CultureMech_002021 --> ing10_Na_DL_lactate
    CHEBI_75228["CHEBI:75228"]
    ing10_Na_DL_lactate -.-> CHEBI_75228
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002021 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_HCl["HCl"]
    CultureMech_002021 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002021 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_ZnCl2["ZnCl2"]
    CultureMech_002021 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002021 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_002021 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002021 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002021 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002021 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002021 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_NaOH["NaOH"]
    CultureMech_002021 --> ing21_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing21_NaOH -.-> CHEBI_32145
    ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002021 --> ing22_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002021 --> ing23_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing24_Biotin["Biotin"]
    CultureMech_002021 --> ing24_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_Biotin -.-> CHEBI_15956
    ing25_Folic_acid["Folic acid"]
    CultureMech_002021 --> ing25_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_acid -.-> CHEBI_27470
    ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002021 --> ing26_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing27_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002021 --> ing27_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing27_Thiamine_HCl -.-> CHEBI_49105
    ing28_Riboflavin["Riboflavin"]
    CultureMech_002021 --> ing28_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing28_Riboflavin -.-> CHEBI_17015
    ing29_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002021 --> ing29_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing29_Nicotinic_acid -.-> CHEBI_15940
    CultureMech_002021 --> more["...5 more"]

Ingredients (35)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
21.0084 G_PER_L CHEBI:26710
sodium chloride
2 Na2SO4
2Na.O4S 142.043 g/mol
4.20168 G_PER_L CHEBI:32149
sodium sulfate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.210084 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.262605 G_PER_L CHEBI:31206
ammonium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3.15126 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.157563 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 KBr
Br.K 119.002 g/mol
0.0945378 G_PER_L CHEBI:32030
potassium bromide
8 KCl
Cl.K 74.551 g/mol
0.52521 G_PER_L CHEBI:32588
potassium chloride
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00052521 G_PER_L CHEBI:8806
Resazurin
10 Na2CO3
CO3.2Na 105.988 g/mol
50 G_PER_L CHEBI:29377
sodium carbonate
11 Na-DL-lactate
C3H5O3.Na 112.06 g/mol
250 G_PER_L CHEBI:75228
sodium lactate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
30 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
14 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 NaOH
HO.Na 39.997 g/mol
1.0 G_PER_L CHEBI:32145
sodium hydroxide
 [Merged 2 duplicates: 0.5, 0.5]
23 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
24 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
25 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
26 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
27 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
28 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
29 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
30 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
31 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
32 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
33 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
34 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
35 Na2S2O4
2Na.O4S2 174.11 g/mol
50 G_PER_L CHEBI:66870
sodium dithionite

Preparation

  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 – 45 min), then dispensed under the same gas atmosphere into serum vials (use at least bottles of 50 ml volume filled with 30 ml medium) and autoclaved. Solutions B and F are prepared under 80% N2 and 20% CO2 gas atmosphere and sterilized. Sterile solutions C, D and E are prepared under 100% N2 gas. Solutions D and F are sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 - 7.2.
  2. Note: For transfer of cultures use at least 10% (v/v) inoculum.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  4. Add 1 N NaOH to distilled water and make the solution anoxic by sparging with 100% N2 gas. Then dissolve the Na-dithionite and filter-sterilize the solution into anoxic Hungate tubes. The final pH of the solution should be between 9 and 10. Store the prepared solution in a dark and cool place. Prepare only small amounts of stock solution, as Na-dithionite decomposes rapidly.

Target organisms

No target organisms are recorded.

Provenance

Merged from 7 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:12.545550Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 861a
2026-02-06T05:10:43.298643Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:26.795411Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:09.483491Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002021
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.050336+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOTALEA PSYCHROPHILA MEDIUM" to "desulfotalea_psychrophila_medium" for consistent matching
2026-05-20T06:01:14.478996+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:40:57.387714+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.871922+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:31.342599+00:00 recipe-merger Merged 7 duplicate recipes into canonical record Sources: desulfofrigus_medium, desulfotalea_psychrophila_medium, for_dsm_12341, for_dsm_12342_dsm_12343_and_dsm_12345, for_dsm_12343, for_dsm_12344, srb_psychrophile_medium
2026-08-06T03:05:31.342720+00:00 merge_recipes.py MERGED_RECIPES merged 7 source recipe(s) on fingerprint 7bbceeea99b1cede09e8fff4d46758c7fb6720d77111920be0833e47a92e6447