Overview
- External ontology
- mediadive.medium:86a (source link unavailable) — MODIFIED CASTENHOLZ MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium86a.pdf
Composition
flowchart LR
CultureMech_002027["modified_castenholz_medium"]
ing0_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002027 --> ing0_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing0_Nitrilotriacetic_acid -.-> CHEBI_44557
ing1_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_002027 --> ing1_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing1_CaSO4_x_2_H2O -.-> CHEBI_32583
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002027 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaCl["NaCl"]
CultureMech_002027 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KNO3["KNO3"]
CultureMech_002027 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_NaNO3["NaNO3"]
CultureMech_002027 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_002027 --> ing6_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002027 --> ing7_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
ing8_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_002027 --> ing8_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing8_MnSO4_x_H2O -.-> CHEBI_86364
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002027 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_H3BO3["H3BO3"]
CultureMech_002027 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002027 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002027 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002027 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_Peptone["Peptone"]
CultureMech_002027 --> ing14_Peptone
ing15_Yeast_extract["Yeast extract"]
CultureMech_002027 --> ing15_Yeast_extract
ing16_Monosodium_glutamate["Monosodium glutamate"]
CultureMech_002027 --> ing16_Monosodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing16_Monosodium_glutamate -.-> CHEBI_64220
ing17_Agar["Agar"]
CultureMech_002027 --> ing17_Agar
CHEBI_2509["CHEBI:2509"]
ing17_Agar -.-> CHEBI_2509
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.1 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 2 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.06 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.008 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
KNO3
K.NO3 101.102 g/mol
|
0.103 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.689 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 7 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.14 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 8 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.00047 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 9 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.0022 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0005 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.0005 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
2.5e-05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
2.5e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
4.6e-05 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 | Peptone | 3 G_PER_L | ||
| 16 | Yeast extract | 1 G_PER_L | ||
| 17 |
Monosodium glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 18 | Agar | 25 G_PER_L |
CHEBI:2509
agar
|
(if required) |
Preparation
- Adjust pH to 7.8 with NaOH.
- Solid media is prepared by the addition of 2 - 2.5% agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- DSMZ_86a_MODIFIED_CASTENHOLZ_MEDIUM (CultureMech:002027)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.134374Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 86a |
| 2026-03-13T06:19:09.550728Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002027 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.124078+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED CASTENHOLZ MEDIUM" to "modified_castenholz_medium" for consistent matching |
| 2026-06-05T07:40:16.321443+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.064044+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:42.672096+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:34.917136+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 34d9d01b9c1ffb86b0701b68322437068221393c4e955e0d9751bb987f5f241b |