heliorestis_medium

CultureMech:002046 bacterial COMPLEX LIQUID
Original name: HELIORESTIS MEDIUM

Overview

External ontology
mediadive.medium:886 (source link unavailable) — HELIORESTIS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 9.0, 'max': 9.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium886.pdf

Composition

flowchart LR
    CultureMech_002046["heliorestis_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002046 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002046 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_NaCl["NaCl"]
    CultureMech_002046 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002046 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_CaCl2["CaCl2"]
    CultureMech_002046 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_Na2CO3["Na2CO3"]
    CultureMech_002046 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002046 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Na_acetate["Na-acetate"]
    CultureMech_002046 --> ing7_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Na_acetate -.-> CHEBI_32954
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002046 --> ing8_Yeast_extract
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002046 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_002046 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_Biotin["Biotin"]
    CultureMech_002046 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Resazurin["Resazurin"]
    CultureMech_002046 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002046 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002046 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_002046 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002046 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002046 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002046 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002046 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
3 NaCl
Cl.Na 58.443 g/mol
0.5 G_PER_L CHEBI:26710
sodium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 CaCl2
Ca.2Cl 110.984 g/mol
0.1 G_PER_L CHEBI:3312
calcium dichloride
6 Na2CO3
CO3.2Na 105.988 g/mol
2.5 G_PER_L CHEBI:29377
sodium carbonate
7 NaHCO3
CHO3.Na 84.006 g/mol
2.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Na-acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
9 Yeast extract 0.5 G_PER_L
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.4 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Vitamin B12 2e-05 G_PER_L CHEBI:176843
vitamin B12
12 Biotin
C10H16N2O3S 244.316 g/mol
2e-05 G_PER_L CHEBI:15956
biotin
13 Resazurin
C12H7NO4 229.191 g/mol
0.02 G_PER_L CHEBI:8806
Resazurin
14 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min.
  2. Prepare NaHCO3, Na2CO3 and sulfide as separate sterile stock solution, Vitamin B12 and Biotin as filter-sterilized stock solution and add to sterile medium after autoclaving. Adjust pH to 9 - 9.5 .
  3. To control anoxic conditions, add resazurin to a final concentration of 0.002%.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:12.620972Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 886
2026-03-13T06:19:09.750547Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002046
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.345837+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HELIORESTIS MEDIUM" to "heliorestis_medium" for consistent matching
2026-05-20T06:01:22.482065+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '9-9.5' -> {'min': 9.0, 'max': 9.5}
2026-06-05T07:41:08.377401+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.719486+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:37.475008+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 8babd30381c47d0c95166c2eca0734057ba3eeb8329517ab6cabf8ac8076ab3c