os_medium

CultureMech:002047 bacterial COMPLEX LIQUID
Original name: OS MEDIUM

Overview

External ontology
mediadive.medium:887 (source link unavailable) — OS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium887.pdf

Composition

flowchart LR
    CultureMech_002047["os_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002047 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002047 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002047 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_H3BO3["H3BO3"]
    CultureMech_002047 --> ing3_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing3_H3BO3 -.-> CHEBI_33118
    ing4_Na2SO4["Na2SO4"]
    CultureMech_002047 --> ing4_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing4_Na2SO4 -.-> CHEBI_32149
    ing5_NaNO3["NaNO3"]
    CultureMech_002047 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_KCl["KCl"]
    CultureMech_002047 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002047 --> ing7_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing8_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_002047 --> ing8_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing8_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing9_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002047 --> ing9_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing9_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing10_NaHCO3["NaHCO3"]
    CultureMech_002047 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002047 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002047 --> ing12_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing13_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_002047 --> ing13_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing13_MnSO4_x_H2O -.-> CHEBI_86364
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002047 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002047 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002047 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002047 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002047 --> ing18_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002047 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002047 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002047 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
1.256 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.256, 1.0]
2 KH2PO4
H2O4P.K 136.084 g/mol
0.0017 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1008 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0008, 0.1]
4 H3BO3
H3BO3 61.833 g/mol
0.0203 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.0103, 0.01]
5 Na2SO4
2Na.O4S 142.043 g/mol
0.023 G_PER_L CHEBI:32149
sodium sulfate
6 NaNO3
NO3.Na 84.994 g/mol
0.0003 G_PER_L CHEBI:63005
sodium nitrate
7 KCl
Cl.K 74.551 g/mol
0.015 G_PER_L CHEBI:32588
potassium chloride
8 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.0001 G_PER_L CHEBI:86254
iron trichloride hexahydrate
9 MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
6e-05 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
10 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
11 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
14 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Sparge medium with 100% N2 gas mixture for 30 min to make anoxic.
  2. Adjust pH to 7.0 with H2SO4.
  3. Fill 10 ml medium into 100 ml Serum bottles under a stream of H2/CO2 gas and seal with a rubber stopper.
  4. Autoclave at 121 °C for 20 min.
  5. After autoclaving add the following (e.g., from sterile stock solutions)
  6. Adjust the gas atmosphere strain specifically.
  7. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:12.624075Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 887
2026-03-13T03:09:32.175086Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:09.761871Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002047
2026-03-17T06:30:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.358315+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "OS MEDIUM" to "os_medium" for consistent matching
2026-06-05T07:41:40.618178+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.808431+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:52.074725+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6fbfc90447c8a12b3fb0f0481c303888ddb39b26352ec28d95d6e9d3f7383ee1