Overview
- External ontology
- mediadive.medium:898b (source link unavailable) — SERPENTINICELLA MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'for_dsm_18346_and_dsm_18979', 'source': 'KOMODO', 'source_id': 'komodo.medium:898.3', 'original_category': 'bacterial'}
- {'name': 'for_dsm_18978', 'source': 'KOMODO', 'source_id': 'komodo.medium:898.4', 'original_category': 'bacterial'}
- pH range
- {'min': 9.5, 'max': 9.7}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium898b.pdf
Composition
flowchart LR
CultureMech_002063["serpentinicella_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002063 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002063 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KCl["KCl"]
CultureMech_002063 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_NH4Cl["NH4Cl"]
CultureMech_002063 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaCl["NaCl"]
CultureMech_002063 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002063 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_002063 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_Na2CO3["Na2CO3"]
CultureMech_002063 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
ing8_NaHCO3["NaHCO3"]
CultureMech_002063 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Yeast_extract["Yeast extract"]
CultureMech_002063 --> ing9_Yeast_extract
ing10_Na_crotonate["Na-crotonate"]
CultureMech_002063 --> ing10_Na_crotonate
CHEBI_35899["CHEBI:35899"]
ing10_Na_crotonate -.-> CHEBI_35899
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002063 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_HCl["HCl"]
CultureMech_002063 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002063 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_ZnCl2["ZnCl2"]
CultureMech_002063 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002063 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_H3BO3["H3BO3"]
CultureMech_002063 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002063 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002063 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002063 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002063 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_Biotin["Biotin"]
CultureMech_002063 --> ing21_Biotin
CHEBI_15956["CHEBI:15956"]
ing21_Biotin -.-> CHEBI_15956
ing22_Folic_acid["Folic acid"]
CultureMech_002063 --> ing22_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing22_Folic_acid -.-> CHEBI_27470
ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002063 --> ing23_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing24_Thiamine_HCl["Thiamine HCl"]
CultureMech_002063 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Riboflavin["Riboflavin"]
CultureMech_002063 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Nicotinic_acid["Nicotinic acid"]
CultureMech_002063 --> ing26_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_acid -.-> CHEBI_15940
ing27_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_002063 --> ing27_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing27_Calcium_D_____pantothenate -.-> CHEBI_31345
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_002063 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002063 --> ing29_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
CultureMech_002063 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.199601 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0998004 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.199601 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.998004 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.998004 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.49701 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000499002 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2.75449 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
9.98004 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 | Yeast extract | 4.99002 G_PER_L | ||
| 11 |
Na-crotonate
C4H5O2 85.082 g/mol
|
1.71657 G_PER_L |
CHEBI:35899
crotonate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.499002 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 23 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 24 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 25 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 27 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 28 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 29 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 30 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 31 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve all ingredients except carbonates, yeast extract, crotonate, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate and hydrogencarbonate, adjust pH to 9.0 - 9.5, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, crotonate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 9.5 - 9.7.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- for_dsm_18346_and_dsm_18979 (CultureMech:006756)
- for_dsm_18978 (CultureMech:006757)
- serpentinicella_medium (CultureMech:002063)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.671224Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 898b |
| 2026-02-06T05:10:49.546744Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:09.939713Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002063 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.549241+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SERPENTINICELLA MEDIUM" to "serpentinicella_medium" for consistent matching |
| 2026-05-20T06:02:05.145262+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '9.5-9.7' -> {'min': 9.5, 'max': 9.7} |
| 2026-06-05T07:41:45.729826+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.449680+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:35.088006+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: for_dsm_18346_and_dsm_18979, for_dsm_18978, serpentinicella_medium |
| 2026-08-06T03:05:35.088061+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 6d9eed3a1abdd4be0324c40af635d6065ff24b7b30e50b75c74c28f75df50a56 |