marinitoga_medium

CultureMech:002071 bacterial COMPLEX LIQUID
Original name: MARINITOGA MEDIUM

Overview

External ontology
mediadive.medium:904 (source link unavailable) — MARINITOGA MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium904.pdf

Composition

flowchart LR
    CultureMech_002071["marinitoga_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_002071 --> ing0_Sea_Salt
    ing1_PIPES["PIPES"]
    CultureMech_002071 --> ing1_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing1_PIPES -.-> CHEBI_39033
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002071 --> ing2_Yeast_extract
    ing3_Tryptone["Tryptone"]
    CultureMech_002071 --> ing3_Tryptone
    ing4_Sodium_resazurin["Sodium resazurin"]
    CultureMech_002071 --> ing4_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Sodium_resazurin -.-> CHEBI_8806
    ing5_D_Glucose["D-Glucose"]
    CultureMech_002071 --> ing5_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing5_D_Glucose -.-> CHEBI_17634
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002071 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002071 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Sea Salt 30 G_PER_L
2 PIPES 6 G_PER_L CHEBI:39033
PIPES
3 Yeast extract 1 G_PER_L
4 Tryptone 1 G_PER_L
5 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
6 D-Glucose
C6H12O6 180.156 g/mol
2.5 G_PER_L CHEBI:17634
D-glucose
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate

Preparation

  1. Dissolve ingredients except glucose, sulfide and cysteine, adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:12.701757Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 904
2026-03-13T06:19:10.028783Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002071
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.649020+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MARINITOGA MEDIUM" to "marinitoga_medium" for consistent matching
2026-06-05T07:41:13.145849+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:42.164809+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6731fa1ed7049024a733960cead1012c6f495ee845b96f156e218dbfd32b4b6c