Overview
- External ontology
- mediadive.medium:906 (source link unavailable) — DETHIOSULFOVIBRIO MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'dethiosulfovibrio_ii_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:906', 'original_category': 'bacterial'}
- pH range
- {'min': 6.7, 'max': 6.8}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium906.pdf
Composition
flowchart LR
CultureMech_002074["dethiosulfovibrio_medium"]
ing0_NaCl["NaCl"]
CultureMech_002074 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002074 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KH2PO4["KH2PO4"]
CultureMech_002074 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Na3_citrate_x_5_H2O["Na3-citrate x 5 H2O"]
CultureMech_002074 --> ing3_Na3_citrate_x_5_H2O
ing4_Yeast_extract["Yeast extract"]
CultureMech_002074 --> ing4_Yeast_extract
ing5_Peptone["Peptone"]
CultureMech_002074 --> ing5_Peptone
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_002074 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002074 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002074 --> ing8_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002074 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_HCl["HCl"]
CultureMech_002074 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002074 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_ZnCl2["ZnCl2"]
CultureMech_002074 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002074 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_002074 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002074 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002074 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002074 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002074 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
19.98 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.997 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.999001 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 | Na3-citrate x 5 H2O | 2.997 G_PER_L | ||
| 5 | Yeast extract | 4.99501 G_PER_L | ||
| 6 | Peptone | 1.998 G_PER_L | ||
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0004995 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1998 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.4975 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.499501 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Dissolve ingredients except calcium chloride, thiosulfate and sulfide. Sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add calcium chloride, thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be at 6.7 - 6.8.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_906_DETHIOSULFOVIBRIO_II_medium (CultureMech:006777)
- dethiosulfovibrio_medium (CultureMech:002074)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.709222Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 906 |
| 2026-03-13T06:19:10.053920Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002074 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.676715+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DETHIOSULFOVIBRIO MEDIUM" to "dethiosulfovibrio_medium" for consistent matching |
| 2026-05-20T06:01:15.482478+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.7-6.8' -> {'min': 6.7, 'max': 6.8} |
| 2026-06-05T07:40:58.607174+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.344847+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:01.682404+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_906_DETHIOSULFOVIBRIO_II_medium, dethiosulfovibrio_medium |
| 2026-08-06T03:05:01.682446+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint a808c7967e9e51ef9b4ed398b3976c5d31e367d1059d22a5c10f07342e730aae |