methanofollis_mb_medium_5_g_l_nacl

CultureMech:002095 archaea COMPLEX LIQUID
Original name: METHANOFOLLIS MB MEDIUM (5 g/l NaCl)

Overview

External ontology
mediadive.medium:924a (source link unavailable) — METHANOFOLLIS MB MEDIUM (5 g/l NaCl)
Applications
  • Microbial cultivation
pH range
{'min': 6.5, 'max': 6.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium924a.pdf

Composition

flowchart LR
    CultureMech_002095["methanofollis_mb_medium_5_g_l_nacl"]
    ing0_NaCl["NaCl"]
    CultureMech_002095 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002095 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KCl["KCl"]
    CultureMech_002095 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002095 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002095 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002095 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002095 --> ing6_Yeast_extract
    ing7_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002095 --> ing7_Trypticase_peptone
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_002095 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_002095 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na_formate["Na-formate"]
    CultureMech_002095 --> ing10_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing10_Na_formate -.-> CHEBI_62965
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002095 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002095 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002095 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002095 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_002095 --> ing15_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing15_MnSO4_x_H2O -.-> CHEBI_86364
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002095 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002095 --> ing17_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002095 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002095 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002095 --> ing20_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing20_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing21_H3BO3["H3BO3"]
    CultureMech_002095 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002095 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002095 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002095 --> ing24_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002095 --> ing25_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing26_Biotin["Biotin"]
    CultureMech_002095 --> ing26_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing26_Biotin -.-> CHEBI_15956
    ing27_Folic_acid["Folic acid"]
    CultureMech_002095 --> ing27_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing27_Folic_acid -.-> CHEBI_27470
    ing28_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002095 --> ing28_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing28_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing29_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002095 --> ing29_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing29_Thiamine_HCl -.-> CHEBI_49105
    CultureMech_002095 --> more["...6 more"]

Ingredients (36)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
5.9456 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 4.9456, 1.0]
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.98912 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KCl
Cl.K 74.551 g/mol
0.49456 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.495648 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.395648, 0.1]
5 K2HPO4
HO4P.2K 174.174 g/mol
0.395648 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.98912 G_PER_L CHEBI:31206
ammonium chloride
7 Yeast extract 1.97824 G_PER_L
8 Trypticase peptone 1.97824 G_PER_L
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00049456 G_PER_L CHEBI:8806
Resazurin
10 NaHCO3
CHO3.Na 84.006 g/mol
3.95648 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Na-formate
CHO2.Na 68.007 g/mol
6.72601 G_PER_L CHEBI:62965
sodium formate
12 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.24728 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.24728 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
16 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
18 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
22 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
25 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
26 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
27 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
28 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
29 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
30 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
31 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
32 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
33 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
34 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
35 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
36 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve ingredients (except bicarbonate, vitamins, formate and reducing agents), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add solid bicarbonate, adjust pH to 6.8 - 7.0, dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins (sterilized by filtration), formate, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be 6.5 - 6.8.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:12.768804Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 924a
2026-02-06T05:10:26.337721Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:59.772398Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:10.246560Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002095
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.893534+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOFOLLIS MB MEDIUM (5 g/l NaCl)" to "methanofollis_mb_medium_5_g_l_nacl" for consistent matching
2026-05-20T06:01:55.616918+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.5-6.8' -> {'min': 6.5, 'max': 6.8}
2026-06-05T07:41:31.434250+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.941383+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:30.352891+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ffa5915f49356f25b306ee99a3b5d28f5f1ef642aa08e8196e6a9fc8dce12e73