Overview
- External ontology
- mediadive.medium:92a (source link unavailable) — MODIFIED MEDIUM 92
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92a.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_002102["modified_medium_92"]
ing0_Biotin["Biotin"]
CultureMech_002102 --> ing0_Biotin
CHEBI_15956["CHEBI:15956"]
ing0_Biotin -.-> CHEBI_15956
ing1_Folic_acid["Folic acid"]
CultureMech_002102 --> ing1_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing1_Folic_acid -.-> CHEBI_27470
ing2_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002102 --> ing2_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing2_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing3_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_002102 --> ing3_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing3_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing4_Riboflavin["Riboflavin"]
CultureMech_002102 --> ing4_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing4_Riboflavin -.-> CHEBI_17015
ing5_Nicotinic_acid["Nicotinic acid"]
CultureMech_002102 --> ing5_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing5_Nicotinic_acid -.-> CHEBI_15940
ing6_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002102 --> ing6_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing6_Calcium_pantothenate -.-> CHEBI_31345
ing7_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002102 --> ing7_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing7_p_Aminobenzoic_acid -.-> CHEBI_30753
ing8_Vitamin_B12["Vitamin B12"]
CultureMech_002102 --> ing8_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing8_Vitamin_B12 -.-> CHEBI_176843
ing9_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_002102 --> ing9_Pancreatic_digest_of_casein
ing10_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_002102 --> ing10_Peptic_digest_of_soybean_meal
ing11_Glucose["Glucose"]
CultureMech_002102 --> ing11_Glucose
CHEBI_17234["CHEBI:17234"]
ing11_Glucose -.-> CHEBI_17234
ing12_Sodium_chloride["Sodium chloride"]
CultureMech_002102 --> ing12_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing12_Sodium_chloride -.-> CHEBI_26710
ing13_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_002102 --> ing13_Dipotassium_phosphate
CHEBI_131527["CHEBI:131527"]
ing13_Dipotassium_phosphate -.-> CHEBI_131527
ing14_Agar["Agar"]
CultureMech_002102 --> ing14_Agar
CHEBI_2509["CHEBI:2509"]
ing14_Agar -.-> CHEBI_2509
ing15_Yeast_extract["Yeast extract"]
CultureMech_002102 --> ing15_Yeast_extract
ing16_Agar["Agar"]
CultureMech_002102 --> ing16_Agar
ing16_Agar -.-> CHEBI_2509
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 2 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 3 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 4 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 5 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 6 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 7 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 8 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.001 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 9 | Vitamin B12 | 1e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 10 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 11 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 12 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 13 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 14 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 15 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 16 | Yeast extract | 3 G_PER_L | ||
| 17 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if necessary) |
Preparation
- Prepare medium 92 and subsequently add vitamins. Adjust pH to 7.0 - 7.2
- Adjust pH to 7.0 - 7.2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_medium_92 (CultureMech:002102)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.157291Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 92a |
| 2026-03-13T06:19:10.325630Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002102 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.984469+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED MEDIUM 92" to "modified_medium_92" for consistent matching |
| 2026-06-05T07:41:37.156826+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.867466+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3de3da748606522447dee61287980f8626f4ae23c1ccb671b0aae25f27e00afe |