Overview
- External ontology
- mediadive.medium:938 (source link unavailable) — ST21 AGAR FOR CELLULOSE DEGRADERS
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium938.pdf
Composition
flowchart LR
CultureMech_002113["st21_agar_for_cellulose_degraders"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002113 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_Yeast_extract["Yeast extract"]
CultureMech_002113 --> ing1_Yeast_extract
ing2_Agar["Agar"]
CultureMech_002113 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Tap_water["Tap water"]
CultureMech_002113 --> ing3_Tap_water
CHEBI_15377["CHEBI:15377"]
ing3_Tap_water -.-> CHEBI_15377
ing4_KNO3["KNO3"]
CultureMech_002113 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002113 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002113 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_MnSO4_x_7_H2O["MnSO4 x 7 H2O"]
CultureMech_002113 --> ing7_MnSO4_x_7_H2O
CHEBI_86360["CHEBI:86360"]
ing7_MnSO4_x_7_H2O -.-> CHEBI_86360
ing8_FeCl3["FeCl3"]
CultureMech_002113 --> ing8_FeCl3
CHEBI_30808["CHEBI:30808"]
ing8_FeCl3 -.-> CHEBI_30808
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.66667 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 | Yeast extract | 0.0333333 G_PER_L | ||
| 3 | Agar | 23.3333 G_PER_L |
CHEBI:2509
agar
|
|
| 4 |
Tap water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
[Merged 3 duplicates: 600.0, 300.0, 100.0] |
| 5 |
KNO3
K.NO3 101.102 g/mol
|
3.33333 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.33333 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.33333 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
MnSO4 x 7 H2O
Mn.O4S 151.001 g/mol
|
0.333333 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 9 |
FeCl3
Cl3Fe 162.204 g/mol
|
2 G_PER_L |
CHEBI:30808
iron trichloride
|
Preparation
- Autoclave solutions separately and combine after cooling to 50°C. A slight precipitate does not restrict agar quality. Adjust pH to 7.2 with KOH using pH indicating paper strips before pouring plates. Place 3-4 strips of filter paper (ca 1 cm2) on the gelated plates and inoculate the filter strips.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- st21_agar_for_cellulose_degraders (CultureMech:002113)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.829686Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 938 |
| 2026-03-13T03:08:49.619272Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:10.439511Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002113 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.105632+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ST21 AGAR FOR CELLULOSE DEGRADERS" to "st21_agar_for_cellulose_degraders" for consistent matching |
| 2026-06-05T07:41:46.888775+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.861100+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:11.797425+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.544189+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2302432714b1ac44c8f6ae4a1f666b1a09e339a813fd2cee88cd44b57ae24c0d |