sedimentibacter_saalensis_medium

CultureMech:002119 bacterial COMPLEX LIQUID
Original name: SEDIMENTIBACTER SAALENSIS MEDIUM

Overview

External ontology
mediadive.medium:943 (source link unavailable) — SEDIMENTIBACTER SAALENSIS MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'zf2_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:943', 'original_category': 'bacterial'}
pH
7.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium943.pdf

Composition

flowchart LR
    CultureMech_002119["sedimentibacter_saalensis_medium"]
    ing0__NH4_HCO3["(NH4)HCO3"]
    CultureMech_002119 --> ing0__NH4_HCO3
    CHEBI_184335["CHEBI:184335"]
    ing0__NH4_HCO3 -.-> CHEBI_184335
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002119 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
    CultureMech_002119 --> ing2_MgSO4_x_6_H2O
    CHEBI_32599["CHEBI:32599"]
    ing2_MgSO4_x_6_H2O -.-> CHEBI_32599
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002119 --> ing3_Yeast_extract
    ing4_NaHCO3["NaHCO3"]
    CultureMech_002119 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Sodium_resazurin["Sodium resazurin"]
    CultureMech_002119 --> ing5_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Sodium_resazurin -.-> CHEBI_8806
    ing6_Sodium_Potassium_phosphate_buffer["Sodium Potassium phosphate buffer"]
    CultureMech_002119 --> ing6_Sodium_Potassium_phosphate_buffer
    ing7_Glycine["Glycine"]
    CultureMech_002119 --> ing7_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing7_Glycine -.-> CHEBI_15428
    ing8_L_Arginine_x_HCl["L-Arginine x HCl"]
    CultureMech_002119 --> ing8_L_Arginine_x_HCl
    CHEBI_31235["CHEBI:31235"]
    ing8_L_Arginine_x_HCl -.-> CHEBI_31235
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002119 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_HCl["HCl"]
    CultureMech_002119 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002119 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_002119 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002119 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_002119 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002119 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002119 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002119 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002119 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NaOH["NaOH"]
    CultureMech_002119 --> ing19_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing19_NaOH -.-> CHEBI_32145
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002119 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002119 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_Vitamin_B12["Vitamin B12"]
    CultureMech_002119 --> ing22_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing22_Vitamin_B12 -.-> CHEBI_176843
    ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002119 --> ing23_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing24_D_____biotin["D-(+)-biotin"]
    CultureMech_002119 --> ing24_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_D_____biotin -.-> CHEBI_15956
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002119 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002119 --> ing26_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002119 --> ing27_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing28_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_002119 --> ing28_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing28_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 (NH4)HCO3
CHO3.H4N 79.055 g/mol
0.45 G_PER_L CHEBI:184335
Ammonium bicarbonate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.12 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 MgSO4 x 6 H2O
Mg.O4S 120.368 g/mol
0.13 G_PER_L CHEBI:32599
magnesium sulfate
4 Yeast extract 3 G_PER_L
5 NaHCO3
CHO3.Na 84.006 g/mol
3.8 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
7 Sodium Potassium phosphate buffer 10 G_PER_L
8 Glycine
C2H5NO2 75.067 g/mol
0.75 G_PER_L CHEBI:15428
glycine
9 L-Arginine x HCl
C6H14N4O2.HCl 210.665 g/mol
2.11 G_PER_L CHEBI:31235
Arginine hydrochloride
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.15 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
24 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
25 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
26 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
27 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
28 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.3 G_PER_L CHEBI:30961
pyridoxine hydrochloride
29 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.2 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. Dissolve ingredients (except bicarbonate, phosphate buffer, glycine, arginine, vitamins, and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and adjust pH to 7.3, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add phosphate buffer, glycine, arginine, vitamins (sterilized by filtration), and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Prior to inoculation add 10 - 20 mg sodium dithionite per liter medium from a 5% (w/v) stock solution, freshly prepared under 100% N2 gas and filter-sterilized.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:12.840541Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 943
2026-01-28T19:48:13.498538Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:10.482424Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002119
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.153949+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SEDIMENTIBACTER SAALENSIS MEDIUM" to "sedimentibacter_saalensis_medium" for consistent matching
2026-06-05T07:41:45.554859+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.386055+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:55.558955+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: sedimentibacter_saalensis_medium, zf2_medium
2026-08-06T03:05:55.559001+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 79f7bd101a1108a4597bcbef4aa73e5821850ff8e20dd58dc60bf0ebf33d9a71