Overview
- External ontology
- mediadive.medium:968 (source link unavailable) — ANAEROBACILLUS ARSENICISELENATIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium968.pdf
Composition
flowchart LR
CultureMech_002149["anaerobacillus_arseniciselenatis_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002149 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgSO4["MgSO4"]
CultureMech_002149 --> ing1_MgSO4
CHEBI_32599["CHEBI:32599"]
ing1_MgSO4 -.-> CHEBI_32599
ing2_NaCl["NaCl"]
CultureMech_002149 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_002149 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KH2PO4["KH2PO4"]
CultureMech_002149 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NaNO3["NaNO3"]
CultureMech_002149 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_Na_DL_lactate["Na-DL-lactate"]
CultureMech_002149 --> ing6_Na_DL_lactate
CHEBI_75228["CHEBI:75228"]
ing6_Na_DL_lactate -.-> CHEBI_75228
ing7_Yeast_extract["Yeast extract"]
CultureMech_002149 --> ing7_Yeast_extract
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_002149 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_Na2CO3["Na2CO3"]
CultureMech_002149 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_NaHCO3["NaHCO3"]
CultureMech_002149 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002149 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002149 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_HCl["HCl"]
CultureMech_002149 --> ing13_HCl
CHEBI_17883["CHEBI:17883"]
ing13_HCl -.-> CHEBI_17883
ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002149 --> ing14_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
ing15_ZnCl2["ZnCl2"]
CultureMech_002149 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002149 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_H3BO3["H3BO3"]
CultureMech_002149 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002149 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002149 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002149 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002149 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NaOH["NaOH"]
CultureMech_002149 --> ing22_NaOH
CHEBI_32145["CHEBI:32145"]
ing22_NaOH -.-> CHEBI_32145
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002149 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002149 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.0998004 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.0249501 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
89.8204 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.149701 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0798403 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
1.2475 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 7 |
Na-DL-lactate
C3H5O3.Na 112.06 g/mol
|
1.69661 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 8 | Yeast extract | 0.199601 G_PER_L | ||
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000499002 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10.5788 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.19162 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.249501 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.249501 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 15 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients (except carbonate, hydrogencarbonate and reducing agents), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.8, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 9.8, if necessary.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- anaerobacillus_arseniciselenatis_medium (CultureMech:002149)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.932226Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 968 |
| 2026-03-13T06:19:10.763306Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002149 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.476157+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROBACILLUS ARSENICISELENATIS MEDIUM" to "anaerobacillus_arseniciselenatis_medium" for consistent matching |
| 2026-06-05T07:40:48.708994+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.102222+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:23.250707+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d80e000935e44550330efa2815528d71846373f84373d3f893d508a68b285253 |