Overview
- External ontology
- mediadive.medium:968b (source link unavailable) — MLMS-1 MEDIUM (ARSENATE)
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'bacillus_selenitireducens_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:968', 'original_category': 'bacterial'}
- {'name': 'mlms_1_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:968b', 'original_category': 'bacterial'}
- pH
- 9.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium968b.pdf
Composition
flowchart LR
CultureMech_002150["mlms_1_medium_arsenate"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002150 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgSO4["MgSO4"]
CultureMech_002150 --> ing1_MgSO4
CHEBI_32599["CHEBI:32599"]
ing1_MgSO4 -.-> CHEBI_32599
ing2_NaCl["NaCl"]
CultureMech_002150 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_002150 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KH2PO4["KH2PO4"]
CultureMech_002150 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_002150 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_Na2CO3["Na2CO3"]
CultureMech_002150 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_NaHCO3["NaHCO3"]
CultureMech_002150 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Na2HAsO4_x_7_H2O["Na2HAsO4 x 7 H2O"]
CultureMech_002150 --> ing8_Na2HAsO4_x_7_H2O
CHEBI_91257["CHEBI:91257"]
ing8_Na2HAsO4_x_7_H2O -.-> CHEBI_91257
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002150 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_HCl["HCl"]
CultureMech_002150 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002150 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_ZnCl2["ZnCl2"]
CultureMech_002150 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002150 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_002150 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002150 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002150 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002150 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002150 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_NaOH["NaOH"]
CultureMech_002150 --> ing19_NaOH
CHEBI_32145["CHEBI:32145"]
ing19_NaOH -.-> CHEBI_32145
ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002150 --> ing20_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002150 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_Biotin["Biotin"]
CultureMech_002150 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_002150 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002150 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_002150 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_002150 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_002150 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_002150 --> ing28_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_D_____pantothenate -.-> CHEBI_31345
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_002150 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
CultureMech_002150 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.0997009 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.0249252 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
59.8205 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.149551 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0797607 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000498504 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10.5683 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.18744 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
Na2HAsO4 x 7 H2O
7H2O.HAsO4.2Na 312.011 g/mol
|
3.09073 G_PER_L |
CHEBI:91257
disodium hydrogenarsenate heptahydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.817547 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 21 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 31 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 32 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except carbonates, arsenate, vitamins and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and hydrogencarbonate, adjust pH to 9.8, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or seruim vials and autoclave. Prior to inoculation, add arsenate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 9.8, if necessary.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- bacillus_selenitireducens_medium (CultureMech:006925)
- mlms_1_medium (CultureMech:006926)
- mlms_1_medium_arsenate (CultureMech:002150)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.935894Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 968b |
| 2026-02-06T05:10:51.118701Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:10.776666Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002150 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.491228+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MLMS-1 MEDIUM (ARSENATE)" to "mlms_1_medium_arsenate" for consistent matching |
| 2026-06-05T07:41:35.208809+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.221860+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.663940+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: bacillus_selenitireducens_medium, mlms_1_medium, mlms_1_medium_arsenate |
| 2026-08-06T03:05:24.663988+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint a1fa2695edf68583fe49d6e88a382d00d8e823d4a25bc51ec52b8bbe16a91e34 |