petrobacter_succinimandens_medium

CultureMech:002161 bacterial COMPLEX LIQUID
Original name: PETROBACTER SUCCINIMANDENS MEDIUM

Overview

External ontology
mediadive.medium:979 (source link unavailable) — PETROBACTER SUCCINIMANDENS MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'for_geococcus_nitratireducens', 'source': 'KOMODO', 'source_id': 'komodo.medium:979.1', 'original_category': 'bacterial'}
  • {'name': 'for_petrobacter_succinimandens', 'source': 'KOMODO', 'source_id': 'komodo.medium:979.2', 'original_category': 'bacterial'}
  • {'name': 'geococcus_nitratireducens_and_petrobacter_succinimandens_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:979', 'original_category': 'bacterial'}
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium979.pdf

Composition

flowchart LR
    CultureMech_002161["petrobacter_succinimandens_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_002161 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002161 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002161 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002161 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_002161 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_002161 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_NH4Cl["NH4Cl"]
    CultureMech_002161 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_Succinic_acid["Succinic acid"]
    CultureMech_002161 --> ing7_Succinic_acid
    CHEBI_15741["CHEBI:15741"]
    ing7_Succinic_acid -.-> CHEBI_15741
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002161 --> ing8_Yeast_extract
    ing9_Biotin["Biotin"]
    CultureMech_002161 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Folic_acid["Folic acid"]
    CultureMech_002161 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002161 --> ing11_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing12_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002161 --> ing12_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_HCl -.-> CHEBI_49105
    ing13_Riboflavin["Riboflavin"]
    CultureMech_002161 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002161 --> ing14_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing14_Nicotinic_acid -.-> CHEBI_15940
    ing15_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_002161 --> ing15_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_002161 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002161 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_002161 --> ing18__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing18__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002161 --> ing19_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing20_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002161 --> ing20_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing20_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing21_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_002161 --> ing21_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing21_MnSO4_x_H2O -.-> CHEBI_86364
    ing22_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002161 --> ing22_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing22_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing23_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002161 --> ing23_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing23_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing24_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002161 --> ing24_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing24_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing25_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002161 --> ing25_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing25_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing26_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002161 --> ing26_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing26_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing27_H3BO3["H3BO3"]
    CultureMech_002161 --> ing27_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing27_H3BO3 -.-> CHEBI_33118
    ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002161 --> ing28_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002161 --> ing29_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
    CultureMech_002161 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.1, 0.1]
5 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
6 NaCl
Cl.Na 58.443 g/mol
2.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 1.0, 1.0]
7 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
8 Succinic acid
C4H6O4 118.088 g/mol
2.36 G_PER_L CHEBI:15741
succinic acid
9 Yeast extract 1 G_PER_L
10 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
11 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
12 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
13 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
14 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
15 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
16 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
17 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
18 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
19 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
20 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
21 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
22 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
23 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
24 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
25 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
26 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
27 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
28 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
29 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
30 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
31 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Prepare the medium without the vitamins.
  2. Adjust pH to 7.0 with 10M KOH.
  3. Autoclave at 121 °C for 20 min.
  4. After cooling add vitamins from a filter sterilised stock solution.
  5. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:12.976580Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 979
2026-02-06T05:10:16.189183Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:44.325307Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:10.872786Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002161
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.597728+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PETROBACTER SUCCINIMANDENS MEDIUM" to "petrobacter_succinimandens_medium" for consistent matching
2026-06-05T07:41:41.440631+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.068193+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:35.506027+00:00 recipe-merger Merged 4 duplicate recipes into canonical record Sources: for_geococcus_nitratireducens, for_petrobacter_succinimandens, geococcus_nitratireducens_and_petrobacter_succinimandens_medium, petrobacter_succinimandens_medium
2026-08-06T03:05:35.506100+00:00 merge_recipes.py MERGED_RECIPES merged 4 source recipe(s) on fingerprint aa021c3184463de00a5e1813a0f70c40d87f97ca6b1c084db016392092b5bfa3