Overview
- External ontology
- mediadive.medium:986 (source link unavailable) — PARAMALEDIVIBACTER MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'clostridium_caminithermalis_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:986', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium986.pdf
Composition
flowchart LR
CultureMech_002168["paramaledivibacter_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_002168 --> ing0_Sea_Salt
ing1_NH4Cl["NH4Cl"]
CultureMech_002168 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_K2HPO4["K2HPO4"]
CultureMech_002168 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KH2PO4["KH2PO4"]
CultureMech_002168 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Peptone["Peptone"]
CultureMech_002168 --> ing4_Peptone
ing5_Yeast_extract["Yeast extract"]
CultureMech_002168 --> ing5_Yeast_extract
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_002168 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_002168 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_D_Glucose["D-Glucose"]
CultureMech_002168 --> ing8_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing8_D_Glucose -.-> CHEBI_17634
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002168 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002168 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_Biotin["Biotin"]
CultureMech_002168 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_002168 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002168 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl["Thiamine HCl"]
CultureMech_002168 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Riboflavin["Riboflavin"]
CultureMech_002168 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_002168 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_002168 --> ing17_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_Calcium_D_____pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_002168 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002168 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_002168 --> ing20__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 29.97 G_PER_L | ||
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.999001 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2997 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2997 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 | Peptone | 0.499501 G_PER_L | ||
| 6 | Yeast extract | 0.499501 G_PER_L | ||
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0004995 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.998 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
D-Glucose
C6H12O6 180.156 g/mol
|
3.996 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.24975 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.24975 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except bicarbonate, glucose, vitamins, cysteine and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- clostridium_caminithermalis_medium (CultureMech:006948)
- paramaledivibacter_medium (CultureMech:002168)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.997407Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 986 |
| 2026-02-06T05:10:43.363070Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:10.935569Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002168 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.666886+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PARAMALEDIVIBACTER MEDIUM" to "paramaledivibacter_medium" for consistent matching |
| 2026-06-05T07:41:40.928130+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.894408+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:27.921182+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: clostridium_caminithermalis_medium, paramaledivibacter_medium |
| 2026-08-06T03:05:27.921232+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint b16d69eb70f443b3fa2d884854e6f573e0c23a5b6fb80f07971ad754642a83d3 |