Overview
- External ontology
- mediadive.medium:994 (source link unavailable) — MINERAL MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'mineral_medium_replace_sorbit_with_d_glucose', 'source': 'KOMODO', 'source_id': 'komodo.medium:994_replace_Sorbit_with_D-Glucose', 'original_category': 'bacterial'}
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium994.pdf
Composition
flowchart LR
CultureMech_002179["mineral_medium"]
ing0_D_Glucose["D-Glucose"]
CultureMech_002179 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_NH4Cl["NH4Cl"]
CultureMech_002179 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KH2PO4["KH2PO4"]
CultureMech_002179 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002179 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_NaCl["NaCl"]
CultureMech_002179 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002179 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_002179 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_Pantothenic_acid["Pantothenic acid"]
CultureMech_002179 --> ing7_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing7_Pantothenic_acid -.-> CHEBI_7916
ing8_Riboflavin["Riboflavin"]
CultureMech_002179 --> ing8_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing8_Riboflavin -.-> CHEBI_17015
ing9_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_002179 --> ing9_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing9_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing10_Biotin["Biotin"]
CultureMech_002179 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_002179 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Nicotinic_acid["Nicotinic acid"]
CultureMech_002179 --> ing12_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing12_Nicotinic_acid -.-> CHEBI_15940
ing13_Nicotine_amide["Nicotine amide"]
CultureMech_002179 --> ing13_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing13_Nicotine_amide -.-> CHEBI_17154
ing14_alpha_lipoic_acid["alpha-lipoic acid"]
CultureMech_002179 --> ing14_alpha_lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing14_alpha_lipoic_acid -.-> CHEBI_16494
ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002179 --> ing15_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
ing16_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_002179 --> ing16_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing16_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing17_Na2_EDTA["Na2-EDTA"]
CultureMech_002179 --> ing17_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing17_Na2_EDTA -.-> CHEBI_64734
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002179 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002179 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002179 --> ing20_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
ing21_H3BO3["H3BO3"]
CultureMech_002179 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002179 --> ing22_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
ing23_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002179 --> ing23_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing23_CuCl2_x_2_H2O -.-> CHEBI_86318
ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002179 --> ing24_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002179 --> ing25_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
3.6 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.6 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0005 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 8 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 9 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 10 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 14 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 15 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 16 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 17 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 18 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 21 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 24 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 25 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 26 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- pH 6.8.
- Stir for some hours, filter sterilize the solution.
- First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- DSMZ_994_MINERAL_MEDIUM (CultureMech:002179)
- KOMODO_994_MINERAL_MEDIUM (CultureMech:006978)
- mineral_medium_replace_sorbit_with_d_glucose (CultureMech:006977)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.020801Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 994 |
| 2026-03-13T06:19:11.000368Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002179 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.740066+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MINERAL MEDIUM" to "mineral_medium" for consistent matching |
| 2026-06-05T07:40:16.396840+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.692279+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.016274+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: DSMZ_994_MINERAL_MEDIUM, KOMODO_994_MINERAL_MEDIUM, mineral_medium_replace_sorbit_with_d_glucose |
| 2026-08-06T03:04:35.016325+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 9e943dcbdeebd6741e8b7fb3a75aae0abd014b2093b995e72e30343fb9170b7a |