Overview
- External ontology
- mediadive.medium:996 (source link unavailable) — PERSEPHONELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium996.pdf
Composition
flowchart LR
CultureMech_002181["persephonella_medium"]
ing0_NaCl["NaCl"]
CultureMech_002181 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NaOH["NaOH"]
CultureMech_002181 --> ing1_NaOH
CHEBI_32145["CHEBI:32145"]
ing1_NaOH -.-> CHEBI_32145
ing2_KCl["KCl"]
CultureMech_002181 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002181 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002181 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002181 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002181 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_NH4Cl["NH4Cl"]
CultureMech_002181 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_002181 --> ing8_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing9_Na_EDTA_x_2_H2O["Na-EDTA x 2 H2O"]
CultureMech_002181 --> ing9_Na_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing9_Na_EDTA_x_2_H2O -.-> CHEBI_64758
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002181 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002181 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002181 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_ZnCl2["ZnCl2"]
CultureMech_002181 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
CultureMech_002181 --> ing14_AlCl3_x_6_H2O
CHEBI_30115["CHEBI:30115"]
ing14_AlCl3_x_6_H2O -.-> CHEBI_30115
ing15_Na2O4W_x_2_H2O["Na2O4W x 2 H2O"]
CultureMech_002181 --> ing15_Na2O4W_x_2_H2O
ing16_CuCl["CuCl"]
CultureMech_002181 --> ing16_CuCl
CHEBI_53472["CHEBI:53472"]
ing16_CuCl -.-> CHEBI_53472
ing17_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_002181 --> ing17_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing17_NiSO4_x_6_H2O -.-> CHEBI_53001
ing18_H2SeO3["H2SeO3"]
CultureMech_002181 --> ing18_H2SeO3
CHEBI_26642["CHEBI:26642"]
ing18_H2SeO3 -.-> CHEBI_26642
ing19_H3BO3["H3BO3"]
CultureMech_002181 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002181 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
29 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
NaOH
HO.Na 39.997 g/mol
|
2 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1.36 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.2 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
Na-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
0.5 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.15 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
|
0.04 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
|
| 16 | Na2O4W x 2 H2O | 0.03 G_PER_L | ||
| 17 |
CuCl
ClCu 98.999 g/mol
|
0.02 G_PER_L |
CHEBI:53472
copper(I) chloride
|
|
| 18 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
0.02 G_PER_L |
CHEBI:53001
nickel sulfate
|
|
| 19 |
H2SeO3
H2O3Se 128.973 g/mol
|
0.01 G_PER_L |
CHEBI:26642
selenous acid
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Medium is prepared with anoxic water (under a CO2-atmosphere) and the pH is adjusted to 6 with H2SO4 prior to autoclaving. Medium is dispensed under a CO2-atmosphere into Bellco tubes (5 ml medium per 27 ml tube) and stoppered with butyl stoppers, capped and crimped. After autoclaving a white precipitate might be present, this precipitate can be redissolved by shaking the medium. It can take up to an hour before all the precipitate is dissolved.
- 3.8% O2 is added to each tube (1 ml of O2 per 27 ml tube) and after inoculation the tubes were pressurized with H2 to 20 psi (or 138 kPa). Inoculum should be in mid-logarithmic phase (16-10 h after inoculation). Incubation is carried out without shaking at 70°C and both strains (DSM 14351 and DSM 14350) grow within 24 h.
- Adjust pH of trace elements to 3.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- persephonella_medium (CultureMech:002181)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.026238Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 996 |
| 2026-03-13T06:19:11.015442Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002181 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.756742+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PERSEPHONELLA MEDIUM" to "persephonella_medium" for consistent matching |
| 2026-06-05T07:41:41.367586+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.040734+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.617945+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fd71a5651967638f633491795b34f00a056721fbdc3ee87725be39f57469aad0 |