Overview
- External ontology
- mediadive.medium:J1000 (source link unavailable) — PYVG MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1000
Composition
flowchart LR
CultureMech_002187["pyvg_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002187 --> ing0_Trypticase_peptone
ing1_Bacto_peptone["Bacto peptone"]
CultureMech_002187 --> ing1_Bacto_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002187 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_002187 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Resazurin["Resazurin"]
CultureMech_002187 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002187 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002187 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002187 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_K2HPO4["K2HPO4"]
CultureMech_002187 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_KH2PO4["KH2PO4"]
CultureMech_002187 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10_NaHCO3["NaHCO3"]
CultureMech_002187 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_NaCl["NaCl"]
CultureMech_002187 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_Acetic_acid["Acetic acid"]
CultureMech_002187 --> ing12_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing12_Acetic_acid -.-> CHEBI_15366
ing13_Propionic_acid["Propionic acid"]
CultureMech_002187 --> ing13_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing13_Propionic_acid -.-> CHEBI_30768
ing14_n_Butyric_acid["n-Butyric acid"]
CultureMech_002187 --> ing14_n_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing14_n_Butyric_acid -.-> CHEBI_30772
ing15_iso_Butyric_acid["iso-Butyric acid"]
CultureMech_002187 --> ing15_iso_Butyric_acid
CHEBI_16135["CHEBI:16135"]
ing15_iso_Butyric_acid -.-> CHEBI_16135
ing16_n_Valeric_acid["n-Valeric acid"]
CultureMech_002187 --> ing16_n_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing16_n_Valeric_acid -.-> CHEBI_17418
ing17_iso_Valeric_acid["iso-Valeric acid"]
CultureMech_002187 --> ing17_iso_Valeric_acid
CHEBI_28484["CHEBI:28484"]
ing17_iso_Valeric_acid -.-> CHEBI_28484
ing18_DL_alpha_Methyl_butyric_acid["DL-alpha-Methyl butyric acid"]
CultureMech_002187 --> ing18_DL_alpha_Methyl_butyric_acid
CHEBI_37070["CHEBI:37070"]
ing18_DL_alpha_Methyl_butyric_acid -.-> CHEBI_37070
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 4.69925 G_PER_L | ||
| 2 | Bacto peptone | 4.69925 G_PER_L | ||
| 3 | Yeast extract | 9.3985 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
9.3985 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.469925 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.48 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 13 |
Acetic acid
C2H4O2 60.052 g/mol
|
17 G_PER_L |
CHEBI:15366
acetic acid
|
|
| 14 |
Propionic acid
C3H6O2 74.079 g/mol
|
6 G_PER_L |
CHEBI:30768
propionic acid
|
|
| 15 |
n-Butyric acid
C4H8O2 88.106 g/mol
|
4 G_PER_L |
CHEBI:30772
butyric acid
|
|
| 16 |
iso-Butyric acid
C4H8O2 88.106 g/mol
|
1 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 17 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 18 |
iso-Valeric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 19 |
DL-alpha-Methyl butyric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:37070
2-methylbutyric acid
|
Preparation
- Adjust pH to 7.0.
- Prepare medium anaerobically under a 100% N2 gas-atmosphere. Dispense in anaerobic vials (1/3 vol.) and autoclave.
- Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pyvg_medium (CultureMech:002187)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:18.272408Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1000 |
| 2026-03-13T06:19:11.071998Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002187 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.817688+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYVG MEDIUM" to "pyvg_medium" for consistent matching |
| 2026-06-05T07:41:43.525832+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.746020+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.034283+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d66d387be540291092c5ff2eed0d69ae568c5e0ec4110b75b498560a974570f6 |