Overview
- External ontology
- mediadive.medium:J1013 (source link unavailable) — ACETIVIBRIO MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1013
Composition
flowchart LR
CultureMech_002198["acetivibrio_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_002198 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Sucrose["Sucrose"]
CultureMech_002198 --> ing1_Sucrose
CHEBI_17992["CHEBI:17992"]
ing1_Sucrose -.-> CHEBI_17992
ing2_Yeast_extract["Yeast extract"]
CultureMech_002198 --> ing2_Yeast_extract
ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002198 --> ing3_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_x_9_H2O -.-> CHEBI_76209
ing4_KH2PO4["KH2PO4"]
CultureMech_002198 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_002198 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002198 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002198 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_Resazurin["Resazurin"]
CultureMech_002198 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_HCl["HCl"]
CultureMech_002198 --> ing9_HCl
CHEBI_17883["CHEBI:17883"]
ing9_HCl -.-> CHEBI_17883
ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002198 --> ing10_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
ing11_ZnCl2["ZnCl2"]
CultureMech_002198 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002198 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_H3BO3["H3BO3"]
CultureMech_002198 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002198 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002198 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002198 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002198 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_NaOH["NaOH"]
CultureMech_002198 --> ing18_NaOH
CHEBI_32145["CHEBI:32145"]
ing18_NaOH -.-> CHEBI_32145
ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002198 --> ing19_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002198 --> ing20_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 2 |
Sucrose
C12H22O11 342.297 g/mol
|
10 G_PER_L |
CHEBI:17992
sucrose
|
|
| 3 | Yeast extract | 0.0499002 G_PER_L | ||
| 4 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.249501 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.47368 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.526316 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.210526 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.157895 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00105263 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 11 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 20 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 21 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Autoclave Basal medium supplemented with Trace element solution SL-10 and Selenite-tungstate solution under 100% N2 gas. Separately autoclave sucrose and yeast extract, and add to the medium. Filter-sterilize Vitamin solution and NaHCO3 solution, and add to the medium. Distribute the medium into culture vessels under 100% N2 gas and seal with butyl rubber stoppers. Before inoculation, reduce the medium with 5% Na2S x 9H2O solution (autoclaved under N2 gas). Readjust pH to 7.7 - 7.9, if necessary.
- Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.
Solutions
Vitamin solution
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1013_ACETIVIBRIO_MEDIUM (CultureMech:002198)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.319549Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1013 |
| 2026-03-09T08:05:09.947926+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:11.173341Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002198 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.932651+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACETIVIBRIO MEDIUM" to "acetivibrio_medium" for consistent matching |
| 2026-05-16T09:02:58.616469+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:16.419330+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.697805+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.042478+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0a8a39e6e8e61f26fd910231941aef7323cdfa1f191de06c53bee10023571ddb |