Overview
- External ontology
- mediadive.medium:J1017 (source link unavailable) — PFENNIG THERMOFILUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1017
Composition
flowchart LR
CultureMech_002200["pfennig_thermofilum_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002200 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_002200 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_KCl["KCl"]
CultureMech_002200 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002200 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002200 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Glucose["Glucose"]
CultureMech_002200 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Yeast_extract["Yeast extract"]
CultureMech_002200 --> ing6_Yeast_extract
ing7_Trypticase_peptone["Trypticase peptone"]
CultureMech_002200 --> ing7_Trypticase_peptone
ing8_Resazurin["Resazurin"]
CultureMech_002200 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002200 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002200 --> ing10_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing10_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002200 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_002200 --> ing12__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing12__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002200 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002200 --> ing14_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing14_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002200 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002200 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_Na2SeO4["Na2SeO4"]
CultureMech_002200 --> ing17_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing17_Na2SeO4 -.-> CHEBI_77775
ing18_H3BO3["H3BO3"]
CultureMech_002200 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002200 --> ing19_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
ing20_Biotin["Biotin"]
CultureMech_002200 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Folic_acid["Folic acid"]
CultureMech_002200 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002200 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_002200 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Riboflavin["Riboflavin"]
CultureMech_002200 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_002200 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002200 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_002200 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002200 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29_Lipoic_acid["Lipoic acid"]
CultureMech_002200 --> ing29_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29_Lipoic_acid -.-> CHEBI_16494
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.321324 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.321324 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.321324 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.321324 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.321324 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
1.94742 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 | Yeast extract | 0.097371 G_PER_L | ||
| 8 | Trypticase peptone | 0.486855 G_PER_L | ||
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00097371 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
6 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.784 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.238 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
0.395 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.033 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.094 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.099 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly, adjust pH to 6.0 and autoclave under a N2 gas atmosphere. Before inoculation, add per liter the following solution (autoclaved and stored under a N2 gas atmosphere):
- Cultivate <I>Desulfurococcus kamchatkensis</I> strain 1221n (=JCM 16383) in the above described medium supplemented with 0.6 g/L (final) yeast extract (without the archaeal culture filtrate). After three days of the cultivation, filtrate the grown culture with 0.22 �m (pore size) filter.
- Comment: For cultivation of JCM 19810, increase amount of yeast extract to 1.0 g/L. Growth may be affected by quality of yeast extract.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pfennig_thermofilum_medium (CultureMech:002200)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.330642Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1017 |
| 2026-01-28T19:48:28.080173Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:11.197134Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002200 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.962569+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PFENNIG THERMOFILUM MEDIUM" to "pfennig_thermofilum_medium" for consistent matching |
| 2026-06-05T07:41:41.544047+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.735508+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:10.108318+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.282344+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fc428703e4ecf80f47ea64c8213dc131d557ca64f043d921e37bbe3e7589df1a |