Overview
- External ontology
- mediadive.medium:J1021 (source link unavailable) — MM4Y MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1021
Composition
flowchart LR
CultureMech_002205["mm4y_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002205 --> ing0_Yeast_extract
ing1_Agar["Agar"]
CultureMech_002205 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_002205 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002205 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002205 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002205 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_NaCl["NaCl"]
CultureMech_002205 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_002205 --> ing7_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing8_KH2PO4["KH2PO4"]
CultureMech_002205 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002205 --> ing9_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002205 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002205 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12_H3BO3["H3BO3"]
CultureMech_002205 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002205 --> ing13_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002205 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_002205 --> ing15_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing15_NiSO4_x_6_H2O -.-> CHEBI_53001
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.05 G_PER_L | ||
| 2 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
39.6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.46 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
55.6 MG_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.147 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
55.51 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
12.93 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.17 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.6 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
0.1 G_PER_L |
CHEBI:53001
nickel sulfate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L.
- Add components to distilled water and bring volume to 1.0 L. Sterilize by autoclaving for storage.
- Sterilize by filtration for storage.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mm4y_medium (CultureMech:002205)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.346445Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1021 |
| 2026-03-13T06:19:11.238383Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002205 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.009315+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MM4Y MEDIUM" to "mm4y_medium" for consistent matching |
| 2026-06-05T07:41:35.231152+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.231956+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.860461+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3a8fd869e24d3fb1dfdc0b212fbf70acdc3c1ac2c342128c566f18d2245a0b64 |