Overview
- External ontology
- mediadive.medium:J1023 (source link unavailable) — PYVL MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1023
Composition
flowchart LR
CultureMech_002207["pyvl_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002207 --> ing0_Trypticase_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002207 --> ing1_Yeast_extract
ing2_L_Lysine["L-Lysine"]
CultureMech_002207 --> ing2_L_Lysine
CHEBI_18019["CHEBI:18019"]
ing2_L_Lysine -.-> CHEBI_18019
ing3_Sodium_resazurin["Sodium resazurin"]
CultureMech_002207 --> ing3_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Sodium_resazurin -.-> CHEBI_8806
ing4_Na2CO3["Na2CO3"]
CultureMech_002207 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002207 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_Biotin["Biotin"]
CultureMech_002207 --> ing6_Biotin
CHEBI_15956["CHEBI:15956"]
ing6_Biotin -.-> CHEBI_15956
ing7_Vitamin_B12["Vitamin B12"]
CultureMech_002207 --> ing7_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing7_Vitamin_B12 -.-> CHEBI_176843
ing8_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002207 --> ing8_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing8_p_Aminobenzoic_acid -.-> CHEBI_30753
ing9_Folic_acid["Folic acid"]
CultureMech_002207 --> ing9_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing9_Folic_acid -.-> CHEBI_27470
ing10_Thiamine_HCl["Thiamine HCl"]
CultureMech_002207 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
ing11_Riboflavin["Riboflavin"]
CultureMech_002207 --> ing11_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing11_Riboflavin -.-> CHEBI_17015
ing12_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_002207 --> ing12_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing12_Pyridoxamine_hydrochloride -.-> CHEBI_131531
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 9.96016 G_PER_L | ||
| 2 | Yeast extract | 4.98008 G_PER_L | ||
| 3 |
L-Lysine
C6H14N2O2 146.19 g/mol
|
4.98008 G_PER_L |
CHEBI:18019
L-lysine
|
|
| 4 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.298805 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 8 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 9 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.03 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 10 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.05 G_PER_L |
CHEBI:27470
folic acid
|
|
| 11 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 12 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 13 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.15 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
Preparation
- Adjust pH to 7.3. Prepare medium anaerobically and pour into rubber stopped tubes under oxygen-free N2-CO2 (95:5).
Solutions
Salt Solution I
Dose: 75 G_PER_L
See mediadive_5691_Salt_Solution_I.yaml for composition
Salt Solution II
Dose: 75 G_PER_L
See mediadive_2571_Salt_Solution_II.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pyvl_medium (CultureMech:002207)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.351595Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1023 |
| 2026-03-09T08:04:35.792846+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:11.250473Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002207 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.023059+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYVL MEDIUM" to "pyvl_medium" for consistent matching |
| 2026-05-16T09:04:30.127915+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:43.535391+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.750110+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.045141+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ef55c4b0390fc9e2177c35ab9051e4b0d6eaa99cb27297e17a47f93561abc651 |