Overview
- External ontology
- mediadive.medium:J1025 (source link unavailable) — PYX MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1025
Composition
flowchart LR
CultureMech_002209["pyx_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002209 --> ing0_Trypticase_peptone
ing1_Bacto_peptone["Bacto peptone"]
CultureMech_002209 --> ing1_Bacto_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002209 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_002209 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Resazurin["Resazurin"]
CultureMech_002209 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002209 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002209 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002209 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_K2HPO4["K2HPO4"]
CultureMech_002209 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_KH2PO4["KH2PO4"]
CultureMech_002209 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10_NaHCO3["NaHCO3"]
CultureMech_002209 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_NaCl["NaCl"]
CultureMech_002209 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 4.80769 G_PER_L | ||
| 2 | Bacto peptone | 4.80769 G_PER_L | ||
| 3 | Yeast extract | 9.61539 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1.92308 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000961538 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.480769 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under a N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense into culture vessels under a N2 gas atmosphere and autoclave. After autoclaving, add glucose from a sterile, anoxic stock solution prepared under a N2 gas atmosphere.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pyx_medium (CultureMech:002209)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.357453Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1025 |
| 2026-03-13T06:19:11.264401Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002209 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.038313+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYX MEDIUM" to "pyx_medium" for consistent matching |
| 2026-06-05T07:41:43.543483+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.753657+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.055022+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 10966f0cc1babd246c291b97a50d010ef617a1f58a72435f7a27dfe46f840ab7 |