Overview
- External ontology
- mediadive.medium:J1033 (source link unavailable) — ST65 MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1033
Composition
flowchart LR
CultureMech_002217["st65_medium"]
ing0_NaCl["NaCl"]
CultureMech_002217 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002217 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_NH4Cl["NH4Cl"]
CultureMech_002217 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_KCl["KCl"]
CultureMech_002217 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_KH2PO4["KH2PO4"]
CultureMech_002217 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002217 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Sulfur["Sulfur"]
CultureMech_002217 --> ing6_Sulfur
CHEBI_26833["CHEBI:26833"]
ing6_Sulfur -.-> CHEBI_26833
ing7_NaHCO3["NaHCO3"]
CultureMech_002217 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_KNO3["KNO3"]
CultureMech_002217 --> ing8_KNO3
CHEBI_63043["CHEBI:63043"]
ing8_KNO3 -.-> CHEBI_63043
ing9_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002217 --> ing9_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing9_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002217 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002217 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002217 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002217 --> ing13_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing13_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002217 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002217 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_Na2SeO4["Na2SeO4"]
CultureMech_002217 --> ing16_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing16_Na2SeO4 -.-> CHEBI_77775
ing17_H3BO3["H3BO3"]
CultureMech_002217 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002217 --> ing18_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
ing19_SrSO4["SrSO4"]
CultureMech_002217 --> ing19_SrSO4
ing20_CrK_SO4_2_x_12_H2O["CrK(SO4)2 x 12 H2O"]
CultureMech_002217 --> ing20_CrK_SO4_2_x_12_H2O
ing21_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
CultureMech_002217 --> ing21_AlCl3_x_6_H2O
CHEBI_30115["CHEBI:30115"]
ing21_AlCl3_x_6_H2O -.-> CHEBI_30115
ing22_BaCl2["BaCl2"]
CultureMech_002217 --> ing22_BaCl2
CHEBI_63317["CHEBI:63317"]
ing22_BaCl2 -.-> CHEBI_63317
ing23_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
CultureMech_002217 --> ing23_Na2SiO3_x_9_H2O
CHEBI_132108["CHEBI:132108"]
ing23_Na2SiO3_x_9_H2O -.-> CHEBI_132108
ing24_KBr["KBr"]
CultureMech_002217 --> ing24_KBr
CHEBI_32030["CHEBI:32030"]
ing24_KBr -.-> CHEBI_32030
ing25_KI["KI"]
CultureMech_002217 --> ing25_KI
CHEBI_8346["CHEBI:8346"]
ing25_KI -.-> CHEBI_8346
ing26_Na2SO4["Na2SO4"]
CultureMech_002217 --> ing26_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing26_Na2SO4 -.-> CHEBI_32149
ing27_Biotin["Biotin"]
CultureMech_002217 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_Folic_acid["Folic acid"]
CultureMech_002217 --> ing28_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing28_Folic_acid -.-> CHEBI_27470
ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002217 --> ing29_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
CultureMech_002217 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
17.4757 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.20388 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.320388 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.320388 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.160194 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0320388 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Sulfur
S 32.067 g/mol
|
4.85437 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.94175 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
KNO3
K.NO3 101.102 g/mol
|
10 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 10 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.0784 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0238 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0166 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.00242 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.00549 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.00017 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.00945 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.00062 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0198 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 20 | SrSO4 | 0.00092 G_PER_L | ||
| 21 | CrK(SO4)2 x 12 H2O | 0.0005 G_PER_L | ||
| 22 |
AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
|
0.00241 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
|
| 23 |
BaCl2
Ba.2Cl 208.234 g/mol
|
0.00208 G_PER_L |
CHEBI:63317
barium chloride
|
|
| 24 |
Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
|
0.0142 G_PER_L |
CHEBI:132108
sodium silicate nonahydrate
|
|
| 25 |
KBr
Br.K 119.002 g/mol
|
0.0119 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 26 |
KI
I.K 166.002 g/mol
|
0.0166 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 27 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.071 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 30 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 31 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 32 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 33 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 34 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 35 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 36 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 37 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components, except sulfur, bring to a boil and cool down under a CO2 (100%) gas stream. While gassing, add the following components to the medium:
- Bubble CO2 for 3 - 4 min and check pH to be 6.5 - 6.8. Readjust pH to the range if necessary. Autoclave under a CO2 gas atmosphere. Add the following solution to the medium (separately autoclaved under N2):
- Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 10 ml of the medium and 0.05 g sulfur in Hungate tubes) under a CO2 stream and seal with butyl rubber stoppers.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- st65_medium (CultureMech:002217)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.389530Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1033 |
| 2026-01-28T19:48:07.754977Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:11.352143Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002217 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.134609+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ST65 MEDIUM" to "st65_medium" for consistent matching |
| 2026-06-05T07:41:46.904059+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.863951+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:11.800613+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.561947+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 23c515716470eac0307d4ce633caef1ed676067341526ed4b30279cdd06f81fa |