Overview
- External ontology
- mediadive.medium:J1039 (source link unavailable) — METHANOBACTERIUM MEDIUM (VII)
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1039 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_002223["methanobacterium_medium_vii"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002223 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002223 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_002223 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_NH4Cl["NH4Cl"]
CultureMech_002223 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002223 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002223 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Calf_brains["Calf brains"]
CultureMech_002223 --> ing6_Calf_brains
ing7_Beef_heart["Beef heart"]
CultureMech_002223 --> ing7_Beef_heart
ing8_Proteose_peptone["Proteose peptone"]
CultureMech_002223 --> ing8_Proteose_peptone
ing9_Dextrose["Dextrose"]
CultureMech_002223 --> ing9_Dextrose
CHEBI_17634["CHEBI:17634"]
ing9_Dextrose -.-> CHEBI_17634
ing10_Sodium_chloride["Sodium chloride"]
CultureMech_002223 --> ing10_Sodium_chloride
ing10_Sodium_chloride -.-> CHEBI_26710
ing11_Disodium_phosphate["Disodium phosphate"]
CultureMech_002223 --> ing11_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing11_Disodium_phosphate -.-> CHEBI_34683
ing12_Proteose_peptone["Proteose peptone"]
CultureMech_002223 --> ing12_Proteose_peptone
ing13_Yeast_extract["Yeast extract"]
CultureMech_002223 --> ing13_Yeast_extract
ing14_Sodium_acetate["Sodium acetate"]
CultureMech_002223 --> ing14_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing14_Sodium_acetate -.-> CHEBI_32954
ing15_Sodium_formate["Sodium formate"]
CultureMech_002223 --> ing15_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing15_Sodium_formate -.-> CHEBI_62965
ing16_NaHCO3["NaHCO3"]
CultureMech_002223 --> ing16_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing16_NaHCO3 -.-> CHEBI_32139
ing17_Resazurin["Resazurin"]
CultureMech_002223 --> ing17_Resazurin
CHEBI_8806["CHEBI:8806"]
ing17_Resazurin -.-> CHEBI_8806
ing18_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002223 --> ing18_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing18_Na2S_x_9_H2O -.-> CHEBI_76209
ing19_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002223 --> ing19_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing19_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_002223 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002223 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Biotin["Biotin"]
CultureMech_002223 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_002223 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002223 --> ing24_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_DL_Calcium_pantothenate -.-> CHEBI_31345
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002223 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_002223 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_HCl["HCl"]
CultureMech_002223 --> ing27_HCl
CHEBI_17883["CHEBI:17883"]
ing27_HCl -.-> CHEBI_17883
ing28_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002223 --> ing28_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing28_FeCl2_x_4_H2O -.-> CHEBI_86249
ing29_ZnCl2["ZnCl2"]
CultureMech_002223 --> ing29_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing29_ZnCl2 -.-> CHEBI_49976
CultureMech_002223 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.488759 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.391007 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.391007 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.391007 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0488759 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00195503 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 8 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 9 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 10 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 11 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 12 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 13 | Proteose peptone | 5.8651 G_PER_L | ||
| 14 | Yeast extract | 1.95503 G_PER_L | ||
| 15 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.977517 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 16 |
Sodium formate
CHO2.Na 68.007 g/mol
|
1.95503 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 17 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.91007 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 18 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000977517 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 19 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 20 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 21 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 25 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.2 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 29 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 30 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 31 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 32 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 33 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 34 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 35 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 36 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components, except NaHCO3 and vitamin solution, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels under a stream of H2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. Prior to inoculation, add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanobacterium_medium_vii (CultureMech:002223)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.411091Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1039 |
| 2026-03-13T06:19:11.408087Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002223 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.197260+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOBACTERIUM MEDIUM (VII)" to "methanobacterium_medium_vii" for consistent matching |
| 2026-06-05T07:41:30.804172+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.697128+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:29.623922+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fe4003e3ca2bca175625f31263da688cd94fb734f6e4449021ed7ab5de176ad9 |