Overview
- External ontology
- mediadive.medium:J1045 (source link unavailable) — FASTIDIOUS ANAEROBE BROTH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1045
Composition
flowchart LR
CultureMech_002229["fastidious_anaerobe_broth_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_002229 --> ing0_Bacto_peptone
ing1_NaCl["NaCl"]
CultureMech_002229 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Starch["Starch"]
CultureMech_002229 --> ing2_Starch
CHEBI_28017["CHEBI:28017"]
ing2_Starch -.-> CHEBI_28017
ing3_NaHCO3["NaHCO3"]
CultureMech_002229 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Glucose["Glucose"]
CultureMech_002229 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002229 --> ing5_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Sodium_pyruvate -.-> CHEBI_50144
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002229 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Sodium_pyrophosphate["Sodium pyrophosphate"]
CultureMech_002229 --> ing7_Sodium_pyrophosphate
CHEBI_71240["CHEBI:71240"]
ing7_Sodium_pyrophosphate -.-> CHEBI_71240
ing8_L_Arginine["L-Arginine"]
CultureMech_002229 --> ing8_L_Arginine
CHEBI_16467["CHEBI:16467"]
ing8_L_Arginine -.-> CHEBI_16467
ing9_Hemin["Hemin"]
CultureMech_002229 --> ing9_Hemin
CHEBI_50385["CHEBI:50385"]
ing9_Hemin -.-> CHEBI_50385
ing10_Dithiothreitol["Dithiothreitol"]
CultureMech_002229 --> ing10_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing10_Dithiothreitol -.-> CHEBI_18320
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 22.9084 G_PER_L | ||
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
4.98008 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 | Starch | 0.996016 G_PER_L |
CHEBI:28017
starch
|
|
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.398406 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.996016 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.996016 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.498008 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Sodium pyrophosphate
4Na.O7P2 265.901 g/mol
|
0.249004 G_PER_L |
CHEBI:71240
sodium diphosphate
|
|
| 9 |
L-Arginine
C6H14N4O2 174.204 g/mol
|
0.996016 G_PER_L |
CHEBI:16467
L-arginine
|
|
| 10 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
0.00996016 G_PER_L |
CHEBI:50385
hemin
|
|
| 11 |
Dithiothreitol
C4H10O2S2 154.256 g/mol
|
4 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
Preparation
- Mix components, adjust pH 7.2 and autoclave. Cool medium under a N2-CO2 (4:1, v/v) gas stream and add anaerobically the following solution (filter-sterilized).
- Aseptically and anaerobically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Readjust pH to 7.0 - 7.2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- fastidious_anaerobe_broth_medium (CultureMech:002229)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.433033Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1045 |
| 2026-03-13T06:19:11.452762Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002229 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.245234+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FASTIDIOUS ANAEROBE BROTH MEDIUM" to "fastidious_anaerobe_broth_medium" for consistent matching |
| 2026-06-05T07:40:59.859210+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:33.866257+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 334c6ed711ea2c1c5fcf56d385ad6ce560ba6d2e15a7ad23b687bb74b8b8b505 |