Overview
- External ontology
- mediadive.medium:J1064 (source link unavailable) — GEOSPOROBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1064
Composition
flowchart LR
CultureMech_002244["geosporobacter_medium"]
ing0_KCl["KCl"]
CultureMech_002244 --> ing0_KCl
CHEBI_32588["CHEBI:32588"]
ing0_KCl -.-> CHEBI_32588
ing1_KH2PO4["KH2PO4"]
CultureMech_002244 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NaCl["NaCl"]
CultureMech_002244 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_NH4Cl["NH4Cl"]
CultureMech_002244 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002244 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002244 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_Yeast_extract["Yeast extract"]
CultureMech_002244 --> ing6_Yeast_extract
ing7_Glucose["Glucose"]
CultureMech_002244 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_NaHCO3["NaHCO3"]
CultureMech_002244 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002244 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Resazurin["Resazurin"]
CultureMech_002244 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Biotin["Biotin"]
CultureMech_002244 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_002244 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002244 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl["Thiamine HCl"]
CultureMech_002244 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Riboflavin["Riboflavin"]
CultureMech_002244 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_002244 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002244 --> ing17_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_Calcium_pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_002244 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002244 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20_Lipoic_acid["Lipoic acid"]
CultureMech_002244 --> ing20_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20_Lipoic_acid -.-> CHEBI_16494
ing21_Na2_EDTA["Na2-EDTA"]
CultureMech_002244 --> ing21_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing21_Na2_EDTA -.-> CHEBI_64734
ing22_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002244 --> ing22_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing22_MgSO4_x_7_H2O -.-> CHEBI_31795
ing23_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002244 --> ing23_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing23_MnSO4_x_n_H2O -.-> CHEBI_86360
ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002244 --> ing24_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
ing25_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002244 --> ing25_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing25_CoCl2_x_6_H2O -.-> CHEBI_53503
ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002244 --> ing26_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing27_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002244 --> ing27_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing27_CuSO4_x_5_H2O -.-> CHEBI_31440
ing28_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002244 --> ing28_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing28_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing29_H3BO3["H3BO3"]
CultureMech_002244 --> ing29_H3BO3
CHEBI_33118["CHEBI:33118"]
ing29_H3BO3 -.-> CHEBI_33118
CultureMech_002244 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KCl
Cl.K 74.551 g/mol
|
0.499002 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.199601 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1.998004 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.998004, 1.0] |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.0499002 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.22980040000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0998004, 0.13] |
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.399202 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 | Yeast extract | 0.998004 G_PER_L | ||
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
0.359281 G_PER_L |
CHEBI:17234
glucose
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.838323 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.499002 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000998004 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 22 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.64 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 23 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 24 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.55 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 25 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 26 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.17 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 27 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 28 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 29 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.018 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 30 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.011 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 32 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
Preparation
- Mix ingredients except NaHCO3, Trace vitamins and Na2S x 9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 7.5 - 8.0.
- Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- geosporobacter_medium (CultureMech:002244)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.493177Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1064 |
| 2026-03-13T03:09:13.939912Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:11.568505Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002244 |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.392878+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GEOSPOROBACTER MEDIUM" to "geosporobacter_medium" for consistent matching |
| 2026-06-05T07:41:06.281335+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.074711+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:36.144492+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9146061e1bf2212b2d7e8ea51abbff7ee97f231a77a2c55b6b6ddcc7bc296d22 |