Overview
- External ontology
- mediadive.medium:J1070 (source link unavailable) — MO-XQ MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1070
Composition
flowchart LR
CultureMech_002251["mo_xq_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002251 --> ing0_Yeast_extract
ing1_NH4Cl["NH4Cl"]
CultureMech_002251 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KH2PO4["KH2PO4"]
CultureMech_002251 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002251 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002251 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_NaCl["NaCl"]
CultureMech_002251 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_NaHCO3["NaHCO3"]
CultureMech_002251 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Resazurin["Resazurin"]
CultureMech_002251 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Glucose["Glucose"]
CultureMech_002251 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002251 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002251 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_Biotin["Biotin"]
CultureMech_002251 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_002251 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002251 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl["Thiamine HCl"]
CultureMech_002251 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Riboflavin["Riboflavin"]
CultureMech_002251 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_002251 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002251 --> ing17_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_DL_Calcium_pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_002251 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002251 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20_Lipoic_acid["Lipoic acid"]
CultureMech_002251 --> ing20_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20_Lipoic_acid -.-> CHEBI_16494
ing21_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002251 --> ing21_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing21_FeCl3_x_6_H2O -.-> CHEBI_86254
ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002251 --> ing22_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002251 --> ing23_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
ing24_ZnCl2["ZnCl2"]
CultureMech_002251 --> ing24_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing24_ZnCl2 -.-> CHEBI_49976
ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002251 --> ing25_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
ing26_AlCl3["AlCl3"]
CultureMech_002251 --> ing26_AlCl3
CHEBI_30114["CHEBI:30114"]
ing26_AlCl3 -.-> CHEBI_30114
ing27_H3BO3["H3BO3"]
CultureMech_002251 --> ing27_H3BO3
CHEBI_33118["CHEBI:33118"]
ing27_H3BO3 -.-> CHEBI_33118
ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002251 --> ing28_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002251 --> ing29_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
CultureMech_002251 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.899281 G_PER_L | ||
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.481115 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.122302 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.74281 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.132194 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
18.3903 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.26619 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000899281 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
6.47482 G_PER_L |
CHEBI:17234
glucose
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0049 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0088 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.0041 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0067 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.0075 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.0025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.0095 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0027 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.0041 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 22 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.27 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 23 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.2 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 24 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.13 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 25 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.14 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 26 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.001 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 27 |
AlCl3
AlCl3 133.341 g/mol
|
0.01 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 28 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 29 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 30 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 31 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.002 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 32 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.003 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Solution A:
- Mix components, except NaHCO3, and adjust pH to 7.5. Bring to a boil for 5-10 sec., then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense 0.9 volume of the medium into culture vessels (e.g., 18 ml of the medium in 50 ml serum bottle) under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight.
- Solution B:
- Adjust pH to 7.5 and autoclave under a N2 atmosphere.
- To complete the medium, add 0.1 volume of solution B to the culture vessels containing solution A. Prior to inoculation, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mo_xq_medium (CultureMech:002251)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.522956Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1070 |
| 2026-03-13T06:19:11.652137Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002251 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.495286+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MO-XQ MEDIUM" to "mo_xq_medium" for consistent matching |
| 2026-06-05T07:41:35.912869+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.421170+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.660837+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2a597e35292f19c215fefeace523227ccf5481a4aa4118350763b54c1d2ebb4e |