Overview
- External ontology
- mediadive.medium:J1081 (source link unavailable) — NATRANAEROARCHAEUM MEDIUM
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1081
Composition
flowchart LR
CultureMech_002261["natranaeroarchaeum_medium"]
ing0_Basal_mineral_NaCl_medium["Basal mineral NaCl medium"]
CultureMech_002261 --> ing0_Basal_mineral_NaCl_medium
ing1_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002261 --> ing1_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing1_Sodium_pyruvate -.-> CHEBI_50144
ing2_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
CultureMech_002261 --> ing2_Trace_vitamins__see_Medium_No__197_
ing3_Yeast_extract["Yeast extract"]
CultureMech_002261 --> ing3_Yeast_extract
ing4_Na2CO3["Na2CO3"]
CultureMech_002261 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_NaHCO3["NaHCO3"]
CultureMech_002261 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_NaCl["NaCl"]
CultureMech_002261 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_K2HPO4["K2HPO4"]
CultureMech_002261 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_KCl["KCl"]
CultureMech_002261 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NH4Cl["NH4Cl"]
CultureMech_002261 --> ing9_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing9_NH4Cl -.-> CHEBI_31206
ing10_MgCl2["MgCl2"]
CultureMech_002261 --> ing10_MgCl2
CHEBI_6636["CHEBI:6636"]
ing10_MgCl2 -.-> CHEBI_6636
ing11_NaOH["NaOH"]
CultureMech_002261 --> ing11_NaOH
CHEBI_32145["CHEBI:32145"]
ing11_NaOH -.-> CHEBI_32145
ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002261 --> ing12_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing13_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002261 --> ing13_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing13_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing14_EDTA["EDTA"]
CultureMech_002261 --> ing14_EDTA
CHEBI_4735["CHEBI:4735"]
ing14_EDTA -.-> CHEBI_4735
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002261 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002261 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002261 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_002261 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002261 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002261 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002261 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002261 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Basal mineral NaCl medium | 250 G_PER_L | ||
| 2 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 3 | Trace vitamins (see Medium No. 197) | 10 G_PER_L | ||
| 4 | Yeast extract | 0.2 G_PER_L | ||
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
46250 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
8750 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
4000 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
250 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
KCl
Cl.K 74.551 g/mol
|
1250 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 11 |
MgCl2
2Cl.Mg 95.211 g/mol
|
1 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 12 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 13 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 14 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 15 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- To complete the medium, aseptically add the following solution (autoclaved or *filter-sterilized):
- Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):
- Adjust pH to 3.6.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- natranaeroarchaeum_medium (CultureMech:002261)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.564028Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1081 |
| 2026-03-13T06:19:11.747130Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002261 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.607201+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NATRANAEROARCHAEUM MEDIUM" to "natranaeroarchaeum_medium" for consistent matching |
| 2026-06-05T07:41:39.182074+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.372144+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.027133+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 65df3e2f99c5fe56429add7580856ff68df3ae66fc2e0c52455dcd9768660f06 |