halalkaliarchaeum_medium

CultureMech:002262 archaea COMPLEX LIQUID
Original name: HALALKALIARCHAEUM MEDIUM

Overview

External ontology
mediadive.medium:J1082 (source link unavailable) — HALALKALIARCHAEUM MEDIUM
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1082

Composition

flowchart LR
    CultureMech_002262["halalkaliarchaeum_medium"]
    ing0_Basal_mineral_NaCl_medium["Basal mineral NaCl medium"]
    CultureMech_002262 --> ing0_Basal_mineral_NaCl_medium
    ing1_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002262 --> ing1_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing1_Sodium_pyruvate -.-> CHEBI_50144
    ing2_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_002262 --> ing2_Trace_vitamins__see_Medium_No__197_
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002262 --> ing3_Yeast_extract
    ing4_Na2CO3["Na2CO3"]
    CultureMech_002262 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002262 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_NaCl["NaCl"]
    CultureMech_002262 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_K2HPO4["K2HPO4"]
    CultureMech_002262 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_KCl["KCl"]
    CultureMech_002262 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NH4Cl["NH4Cl"]
    CultureMech_002262 --> ing9_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing9_NH4Cl -.-> CHEBI_31206
    ing10_MgCl2["MgCl2"]
    CultureMech_002262 --> ing10_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing10_MgCl2 -.-> CHEBI_6636

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Basal mineral NaCl medium 937.5 G_PER_L
2 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
5 G_PER_L CHEBI:50144
sodium pyruvate
3 Trace vitamins (see Medium No. 197) 10 G_PER_L
4 Yeast extract 0.2 G_PER_L
5 Na2CO3
CO3.2Na 105.988 g/mol
46250 G_PER_L CHEBI:29377
sodium carbonate
6 NaHCO3
CHO3.Na 84.006 g/mol
8750 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 NaCl
Cl.Na 58.443 g/mol
4000 G_PER_L CHEBI:26710
sodium chloride
8 K2HPO4
HO4P.2K 174.174 g/mol
250 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
9 KCl
Cl.K 74.551 g/mol
1250 G_PER_L CHEBI:32588
potassium chloride
10 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
11 MgCl2
2Cl.Mg 95.211 g/mol
1 G_PER_L CHEBI:6636
magnesium dichloride

Preparation

  1. To complete the medium, aseptically add the following solution (autoclaved or *filter-sterilized):
  2. Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.567656Z mediadive-import Imported from MediaDive Source: JCM, ID: J1082
2026-03-13T06:19:11.754670Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002262
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.615540+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALALKALIARCHAEUM MEDIUM" to "halalkaliarchaeum_medium" for consistent matching
2026-06-05T07:41:06.997126+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.262138+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:28.578748+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 1aacbaa3e15564ec018394bead1cfe21a6e7e0ca1b146f8485462f2c88634d53