Overview
- External ontology
- mediadive.medium:J1086 (source link unavailable) — PEAT MEDIUM WITH SUCROSE
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1086
Composition
flowchart LR
CultureMech_002266["peat_medium_with_sucrose"]
ing0_Peptone["Peptone"]
CultureMech_002266 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002266 --> ing1_Yeast_extract
ing2_Sucrose["Sucrose"]
CultureMech_002266 --> ing2_Sucrose
CHEBI_17992["CHEBI:17992"]
ing2_Sucrose -.-> CHEBI_17992
ing3_MES["MES"]
CultureMech_002266 --> ing3_MES
CHEBI_39010["CHEBI:39010"]
ing3_MES -.-> CHEBI_39010
ing4_KCl["KCl"]
CultureMech_002266 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_KH2PO4["KH2PO4"]
CultureMech_002266 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_002266 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002266 --> ing7_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
ing8_ZnCl2["ZnCl2"]
CultureMech_002266 --> ing8_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing8_ZnCl2 -.-> CHEBI_49976
ing9_H3BO3["H3BO3"]
CultureMech_002266 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002266 --> ing10_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002266 --> ing11_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002266 --> ing12_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002266 --> ing13_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002266 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002266 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002266 --> ing16_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002266 --> ing17_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.238095 G_PER_L | ||
| 2 | Yeast extract | 0.952381 G_PER_L | ||
| 3 |
Sucrose
C12H22O11 342.297 g/mol
|
1.90476 G_PER_L |
CHEBI:17992
sucrose
|
|
| 4 | MES | 40 G_PER_L |
CHEBI:39010
MES
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.15 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.36 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
2.68 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.024 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 9 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.075 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.019 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 12 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 13 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.344 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 14 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.026 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.556 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.336 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.009 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 18 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
3.446 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Preparation
- Mix components thoroughly. For preparation of solid medium, add 15.0 g/L agar. After autoclaving and cooling (for solid medium, cooling to 55C), add the following solution (filter-sterilized):
- Aseptically distribute the medium into culture vessels and replace the gas phase with a N2-CO2 (4:1, v/v) gas atmosphere.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- peat_medium_with_sucrose (CultureMech:002266)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.582823Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1086 |
| 2026-03-13T06:19:11.795538Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002266 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.663415+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PEAT MEDIUM WITH SUCROSE" to "peat_medium_with_sucrose" for consistent matching |
| 2026-06-05T07:41:41.095196+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.947040+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.420141+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 47d163e7bfe4d4f7381ce5167781199cb7515726f365bb2f2686ddaeb2cf3c55 |