Overview
- External ontology
- mediadive.medium:J1090 (source link unavailable) — MMJS MEDIUM 2
- Applications
-
- Microbial cultivation
- pH
- 6.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1090
Composition
flowchart LR
CultureMech_002270["mmjs_medium_2"]
ing0_NaCl["NaCl"]
CultureMech_002270 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_002270 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002270 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002270 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002270 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_NH4Cl["NH4Cl"]
CultureMech_002270 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_KCl["KCl"]
CultureMech_002270 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002270 --> ing7_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing7_NiCl2_x_6_H2O -.-> CHEBI_34887
ing8_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002270 --> ing8_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing8_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing9_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002270 --> ing9_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing9_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing10__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_002270 --> ing10__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing10__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing11_NaHCO3["NaHCO3"]
CultureMech_002270 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Na2S2O3["Na2S2O3"]
CultureMech_002270 --> ing12_Na2S2O3
CHEBI_132112["CHEBI:132112"]
ing12_Na2S2O3 -.-> CHEBI_132112
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002270 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002270 --> ing14_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing14_MnSO4_x_n_H2O -.-> CHEBI_86360
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002270 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002270 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002270 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002270 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002270 --> ing19_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing19_AlK_SO4_2 -.-> CHEBI_86463
ing20_H3BO3["H3BO3"]
CultureMech_002270 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002270 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_Biotin["Biotin"]
CultureMech_002270 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_002270 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002270 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_002270 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_002270 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_002270 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002270 --> ing28_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_pantothenate -.-> CHEBI_31345
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_002270 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
CultureMech_002270 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
20.6078 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 19.6078, 1.0] |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.137255 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.080392 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.980392, 0.1] |
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.33333 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.33333, 3.0] |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.09804 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.245098 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.323529 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.000490196 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 9 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.000490196 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 10 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
9.80392e-05 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 11 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
0.00980392 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.75 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
Na2S2O3
2Na.O3S2 158.111 g/mol
|
5 G_PER_L |
CHEBI:132112
sodium thiosulfate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 31 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 32 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components and adjust pH to 5.5. Distribute the medium into culture vessels (e.g., 20 ml in 60 ml-serum bottles) under a stream of N2, seal with butyl rubber stoppers and add Autoclave. After cooling, add per liter the following solutions (filter-sterilized):
- Replace the gas phase with N2-CO2-O2 (94:5:1, v/v/v, 100 kPa). Check final pH to be around 6.2.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mmjs_medium_2 (CultureMech:002270)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.592194Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1090 |
| 2026-03-13T03:09:00.927650Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:11.822143Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002270 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.694140+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJS MEDIUM 2" to "mmjs_medium_2" for consistent matching |
| 2026-06-05T07:41:35.659423+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.609666+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:08.343761+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.392563+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4a8526275da92ce718afa64720718f6c0ba4e6d3ce6d1bb689c3d9876e47eda6 |