Overview
- External ontology
- mediadive.medium:J1093 (source link unavailable) — MJ BASAL MEDIUM WITH THIOSULFATE/2
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1093
Composition
flowchart LR
CultureMech_002273["mj_basal_medium_with_thiosulfate_2"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002273 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KNO3["KNO3"]
CultureMech_002273 --> ing1_KNO3
CHEBI_63043["CHEBI:63043"]
ing1_KNO3 -.-> CHEBI_63043
ing2_NaHCO3["NaHCO3"]
CultureMech_002273 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002273 --> ing3_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing3_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing4_NaCl["NaCl"]
CultureMech_002273 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_K2HPO4["K2HPO4"]
CultureMech_002273 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002273 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002273 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002273 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_KCl["KCl"]
CultureMech_002273 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002273 --> ing10_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
ing11_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002273 --> ing11_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing11_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing12_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002273 --> ing12_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing12_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing13_Biotin["Biotin"]
CultureMech_002273 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_Folic_acid["Folic acid"]
CultureMech_002273 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002273 --> ing15_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing16_Thiamine_HCl["Thiamine HCl"]
CultureMech_002273 --> ing16_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing16_Thiamine_HCl -.-> CHEBI_49105
ing17_Riboflavin["Riboflavin"]
CultureMech_002273 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_002273 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002273 --> ing19_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_Calcium_pantothenate -.-> CHEBI_31345
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_002273 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002273 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Lipoic_acid["Lipoic acid"]
CultureMech_002273 --> ing22_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing22_Lipoic_acid -.-> CHEBI_16494
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002273 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002273 --> ing24_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing24_MnSO4_x_n_H2O -.-> CHEBI_86360
ing25_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002273 --> ing25_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing25_FeSO4_x_7_H2O -.-> CHEBI_75836
ing26_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002273 --> ing26_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing26_CoSO4_x_7_H2O -.-> CHEBI_91244
ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002273 --> ing27_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing28_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002273 --> ing28_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing28_CuSO4_x_5_H2O -.-> CHEBI_31440
ing29_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002273 --> ing29_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing29_AlK_SO4_2 -.-> CHEBI_86463
CultureMech_002273 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.123396 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KNO3
K.NO3 101.102 g/mol
|
0.123396 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
15 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
30.703 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 29.703, 1.0] |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138614 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.238614 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.138614, 0.1] |
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.36634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.36634, 3.0] |
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13861 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.326733 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 11 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.00049505 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 12 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00049505 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 13 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00990099 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 16 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 17 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 20 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 21 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 25 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 26 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 27 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 28 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 29 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 30 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 31 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 32 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components thoroughly and autoclave. After cooling, add the following solutions (filter-sterilized):
- Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) and seal with butyl rubber stoppers. Replace the gas phase with a N2-CO2 (80:20, v/v) gas mixture, and pressurize to 200 kPa. Adjust pH to 6.0, if necessary.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mj_basal_medium_with_thiosulfate_2 (CultureMech:002273)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.602112Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1093 |
| 2026-01-28T19:48:19.392388Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:01.342356Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:11.848213Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002273 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.723827+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ BASAL MEDIUM WITH THIOSULFATE/2" to "mj_basal_medium_with_thiosulfate_2" for consistent matching |
| 2026-06-05T07:41:34.927991+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.602394+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:08.119387+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.641596+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e11095224dc0dc229f045a9ba91c132cd5072e7c9a5661f665fe7b5f6185fcd2 |