alkaline_cellulose_agar

CultureMech:002286 bacterial COMPLEX SOLID_AGAR
Original name: ALKALINE CELLULOSE AGAR

Overview

External ontology
mediadive.medium:J110 (source link unavailable) — ALKALINE CELLULOSE AGAR
Applications
  • Microbial cultivation
pH
9.4
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=110

Composition

flowchart LR
    CultureMech_002286["alkaline_cellulose_agar"]
    ing0_Cellulose["Cellulose"]
    CultureMech_002286 --> ing0_Cellulose
    CHEBI_18246["CHEBI:18246"]
    ing0_Cellulose -.-> CHEBI_18246
    ing1_NH4NO3["NH4NO3"]
    CultureMech_002286 --> ing1_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing1_NH4NO3 -.-> CHEBI_63038
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002286 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002286 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2["CaCl2"]
    CultureMech_002286 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_Peptone["Peptone"]
    CultureMech_002286 --> ing5_Peptone
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002286 --> ing6_Yeast_extract
    ing7_Agar["Agar"]
    CultureMech_002286 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Na2CO3["Na2CO3"]
    CultureMech_002286 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Cellulose
(C6H10O5)n.H2O 180.156 g/mol
16.6667 G_PER_L CHEBI:18246
(1->4)-beta-D-glucan
2 NH4NO3
H4N.NO3 80.043 g/mol
2.22222 G_PER_L CHEBI:63038
ammonium nitrate
3 K2HPO4
HO4P.2K 174.174 g/mol
1.11111 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.444444 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2
Ca.2Cl 110.984 g/mol
0.444444 G_PER_L CHEBI:3312
calcium dichloride
6 Peptone 1.11111 G_PER_L
7 Yeast extract 0.555556 G_PER_L
8 Agar 16.6667 G_PER_L CHEBI:2509
agar
9 Na2CO3
CO3.2Na 105.988 g/mol
60 G_PER_L CHEBI:29377
sodium carbonate

Preparation

  1. Adjust pH to 7.0 with 1 N HCl.
  2. Adjust pH to 9.4 with 6% NaHCO3 solution.
  3. After sterilization, mix axenically 900 ml of Solution A and 100 ml of Solution B.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.746057Z mediadive-import Imported from MediaDive Source: JCM, ID: J110
2026-01-28T19:48:13.603269Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:12.003374Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002286
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.903309+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALKALINE CELLULOSE AGAR" to "alkaline_cellulose_agar" for consistent matching
2026-06-05T07:40:47.847039+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.786023+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:22.340917+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 89c9ed0ee4e94200caae4ee0658fe4cd9b12c1e24349f944895a5d3b17a0d525