Overview
- External ontology
- mediadive.medium:J1114 (source link unavailable) — MODIFIED SW-25 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1114
Composition
flowchart LR
CultureMech_002289["modified_sw_25_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_002289 --> ing0_Casamino_acids
ing1_Yeast_extract["Yeast extract"]
CultureMech_002289 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_002289 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002289 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002289 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_KCl["KCl"]
CultureMech_002289 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaBr["NaBr"]
CultureMech_002289 --> ing6_NaBr
CHEBI_63004["CHEBI:63004"]
ing6_NaBr -.-> CHEBI_63004
ing7_NaHCO3["NaHCO3"]
CultureMech_002289 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002289 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 1 G_PER_L | ||
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
195 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
32.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
51 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
NaBr
Br.Na 102.894 g/mol
|
0.6 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Adjust pH to 7.5, and autoclave. For preparation of solid medium, add 20.0 g/L agar.
- Add components to distilled water and bring volume to 1.0 L.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_sw_25_medium (CultureMech:002289)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.679085Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1114 |
| 2026-01-28T19:48:19.289546Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:12.023109Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002289 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:24.925662+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED SW-25 MEDIUM" to "modified_sw_25_medium" for consistent matching |
| 2026-06-05T07:41:37.813167+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.074635+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:08.965869+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:49.857530+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 902fbe893235e7a3a3ebae2fee28a997143e7b27c1fd689d946ed180c4725a13 |