Overview
- External ontology
- mediadive.medium:J1143 (source link unavailable) — ORENIA METALLIREDUCENS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1143
Composition
flowchart LR
CultureMech_002316["orenia_metallireducens_medium"]
ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002316 --> ing0_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
ing1_NaCl["NaCl"]
CultureMech_002316 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KCl["KCl"]
CultureMech_002316 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002316 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_002316 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2SO4["K2SO4"]
CultureMech_002316 --> ing5_K2SO4
CHEBI_32036["CHEBI:32036"]
ing5_K2SO4 -.-> CHEBI_32036
ing6_KH2PO4["KH2PO4"]
CultureMech_002316 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_KBr["KBr"]
CultureMech_002316 --> ing7_KBr
CHEBI_32030["CHEBI:32030"]
ing7_KBr -.-> CHEBI_32030
ing8_Yeast_extract["Yeast extract"]
CultureMech_002316 --> ing8_Yeast_extract
ing9_Resazurin["Resazurin"]
CultureMech_002316 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_NaHCO3["NaHCO3"]
CultureMech_002316 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002316 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_Glucose["Glucose"]
CultureMech_002316 --> ing12_Glucose
CHEBI_17234["CHEBI:17234"]
ing12_Glucose -.-> CHEBI_17234
ing13_Ferric_citrate["Ferric citrate"]
CultureMech_002316 --> ing13_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing13_Ferric_citrate -.-> CHEBI_144421
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002316 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_K2HPO4["K2HPO4"]
CultureMech_002316 --> ing15_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing15_K2HPO4 -.-> CHEBI_131527
ing16_HCl["HCl"]
CultureMech_002316 --> ing16_HCl
CHEBI_17883["CHEBI:17883"]
ing16_HCl -.-> CHEBI_17883
ing17_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002316 --> ing17_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing17_FeCl2_x_4_H2O -.-> CHEBI_86249
ing18_ZnCl2["ZnCl2"]
CultureMech_002316 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002316 --> ing19_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
ing20_H3BO3["H3BO3"]
CultureMech_002316 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002316 --> ing21_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing21_CoCl2_x_6_H2O -.-> CHEBI_53503
ing22_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002316 --> ing22_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2_x_2_H2O -.-> CHEBI_86318
ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002316 --> ing23_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002316 --> ing24_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing25_Biotin["Biotin"]
CultureMech_002316 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Folic_acid["Folic acid"]
CultureMech_002316 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002316 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_002316 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Riboflavin["Riboflavin"]
CultureMech_002316 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_002316 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1.43678 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
19.1571 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.957854 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
5.0478927 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0478927, 5.0] |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.478927 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
K2SO4
2K.O4S 174.259 g/mol
|
0.421456 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
12.835249 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
[Merged 2 duplicates: 0.335249, 12.5] |
| 8 |
KBr
Br.K 119.002 g/mol
|
0.191571 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 9 | Yeast extract | 0.478927 G_PER_L | ||
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000957854 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.91571 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.0296935 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 14 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
20 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 16 |
K2HPO4
HO4P.2K 174.174 g/mol
|
20 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 17 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 18 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 20 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 23 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 24 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 25 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 32 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 33 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 34 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 35 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 36 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 37 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 38 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (80:20, v/v) gas mixture. When the medium is cooled to around 50C, add the following components:
- Continue flushing with the gas mixture until the chemicals are dissolved completely. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (autoclave and stored under a N2 atmosphere):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- orenia_metallireducens_medium (CultureMech:002316)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.786334Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1143 |
| 2026-03-13T03:09:20.556669Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:12.281157Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002316 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.226280+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ORENIA METALLIREDUCENS MEDIUM" to "orenia_metallireducens_medium" for consistent matching |
| 2026-06-05T07:41:40.591656+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 37 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.798760+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.057001+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 31b6e223e9a10c69a8c621b1cead8613d8fc13a03539d1221ae18e4bdfaf2b24 |