Overview
- External ontology
- mediadive.medium:J1160 (source link unavailable) — MOLLICUTES MZ-XQ MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1160
Composition
flowchart LR
CultureMech_002333["mollicutes_mz_xq_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002333 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002333 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002333 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_002333 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaCl["NaCl"]
CultureMech_002333 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Yeast_extract["Yeast extract"]
CultureMech_002333 --> ing5_Yeast_extract
ing6_Glucose["Glucose"]
CultureMech_002333 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Resazurin["Resazurin"]
CultureMech_002333 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_NaHCO3["NaHCO3"]
CultureMech_002333 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002333 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002333 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_Biotin["Biotin"]
CultureMech_002333 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_002333 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002333 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl["Thiamine HCl"]
CultureMech_002333 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Riboflavin["Riboflavin"]
CultureMech_002333 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_002333 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002333 --> ing17_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_DL_Calcium_pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_002333 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002333 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20_Lipoic_acid["Lipoic acid"]
CultureMech_002333 --> ing20_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20_Lipoic_acid -.-> CHEBI_16494
ing21_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002333 --> ing21_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing21_FeCl2_x_4_H2O -.-> CHEBI_86249
ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002333 --> ing22_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002333 --> ing23_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
ing24_ZnCl2["ZnCl2"]
CultureMech_002333 --> ing24_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing24_ZnCl2 -.-> CHEBI_49976
ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002333 --> ing25_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
ing26_H3BO3["H3BO3"]
CultureMech_002333 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002333 --> ing27_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002333 --> ing28_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
ing29_AlCl3["AlCl3"]
CultureMech_002333 --> ing29_AlCl3
CHEBI_30114["CHEBI:30114"]
ing29_AlCl3 -.-> CHEBI_30114
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0960615 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.84246 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.960615 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.480307 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
24.0154 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 | Yeast extract | 0.960615 G_PER_L | ||
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
1.72911 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000960615 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
6 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
6 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0049 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0088 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.0041 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0067 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.0075 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.0025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.0095 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.0271 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0027 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.0041 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 22 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.99 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 23 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.198 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 24 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.13 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 25 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.136 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 26 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0017 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.0062 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 29 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.013 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 30 |
AlCl3
AlCl3 133.341 g/mol
|
0.013 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Preparation
- Mix components and adjust pH to 7.5. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
Solutions
Selenite-tungstate solution
Dose: 1 G_PER_L
See mediadive_5543_Selenite-tungstate_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mollicutes_mz_xq_medium (CultureMech:002333)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.851598Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1160 |
| 2026-03-09T08:05:00.993535+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:12.450284Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002333 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.424593+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MOLLICUTES MZ-XQ MEDIUM" to "mollicutes_mz_xq_medium" for consistent matching |
| 2026-05-16T09:04:26.540943+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:38.141575+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.073008+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.163018+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint aa7ddd0eac5bfdd985ac624aff4c56f285b0fb7a9286dfca8e092082224cc135 |