Overview
- External ontology
- mediadive.medium:J1177 (source link unavailable) — SEDIMENTISPHAERA L21 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1177
Composition
flowchart LR
CultureMech_002348["sedimentisphaera_l21_medium"]
ing0_NaCl["NaCl"]
CultureMech_002348 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002348 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KCl["KCl"]
CultureMech_002348 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Na2SO4["Na2SO4"]
CultureMech_002348 --> ing3_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing3_Na2SO4 -.-> CHEBI_32149
ing4_NH4Cl["NH4Cl"]
CultureMech_002348 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002348 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_K2HPO4["K2HPO4"]
CultureMech_002348 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Resazurin["Resazurin"]
CultureMech_002348 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Glucose["Glucose"]
CultureMech_002348 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_NaHCO3["NaHCO3"]
CultureMech_002348 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002348 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002348 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002348 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002348 --> ing13_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing14_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002348 --> ing14_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing14_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing15_Biotin["Biotin"]
CultureMech_002348 --> ing15_Biotin
CHEBI_15956["CHEBI:15956"]
ing15_Biotin -.-> CHEBI_15956
ing16_Folic_acid["Folic acid"]
CultureMech_002348 --> ing16_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing16_Folic_acid -.-> CHEBI_27470
ing17_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002348 --> ing17_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing17_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing18_Thiamine_HCl["Thiamine HCl"]
CultureMech_002348 --> ing18_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing18_Thiamine_HCl -.-> CHEBI_49105
ing19_Riboflavin["Riboflavin"]
CultureMech_002348 --> ing19_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing19_Riboflavin -.-> CHEBI_17015
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_002348 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002348 --> ing21_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing21_Calcium_pantothenate -.-> CHEBI_31345
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_002348 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002348 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Lipoic_acid["Lipoic acid"]
CultureMech_002348 --> ing24_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing24_Lipoic_acid -.-> CHEBI_16494
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
116.505 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.82524 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
1.45631 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.970874 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.970874 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.38835 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.38835 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000485437 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 15 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 16 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 17 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 18 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 19 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 20 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 23 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized):
- Aseptically and anaerobically, dispense the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
- Readjust pH to 7.5, if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sedimentisphaera_l21_medium (CultureMech:002348)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.912414Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1177 |
| 2026-03-13T06:19:12.597344Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002348 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.598720+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SEDIMENTISPHAERA L21 MEDIUM" to "sedimentisphaera_l21_medium" for consistent matching |
| 2026-06-05T07:41:45.590030+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.399784+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.575609+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7e03fa325296437ea58ee5bf27fa0e01f7f64aca8bc78af22a7f99eeca5f41bc |