Overview
- External ontology
- mediadive.medium:J1179 (source link unavailable) — M MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1179
Composition
flowchart LR
CultureMech_002350["m_medium"]
ing0_KNO3["KNO3"]
CultureMech_002350 --> ing0_KNO3
CHEBI_63043["CHEBI:63043"]
ing0_KNO3 -.-> CHEBI_63043
ing1_KH2PO4["KH2PO4"]
CultureMech_002350 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002350 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaCl["NaCl"]
CultureMech_002350 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_NaHCO3["NaHCO3"]
CultureMech_002350 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Na2CO3["Na2CO3"]
CultureMech_002350 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_Methanol["Methanol"]
CultureMech_002350 --> ing6_Methanol
CHEBI_17790["CHEBI:17790"]
ing6_Methanol -.-> CHEBI_17790
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.22 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
50 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
Methanol
CH4O 32.042 g/mol
|
5 G_PER_L |
CHEBI:17790
methanol
|
Preparation
- After autoclaving, aseptically add filter-sterilized 2 M NaHCO3 solution, 1 M Na2CO3 solution and methanol. Readjust pH to 9.0, if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- m_medium (CultureMech:002350)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.918256Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1179 |
| 2026-03-13T06:19:12.607136Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002350 |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.610426+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M MEDIUM" to "m_medium" for consistent matching |
| 2026-06-05T07:41:12.338146+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.062632+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:00.727713+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:41.436013+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8c67219d452db893b0629592847694eab5d68a1e16bc1d87e76a426aaa57b696 |